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D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signaling
Strigolactones (SLs) are a new class of carotenoid-derived phytohormones essential for developmental processes shaping plant architecture and interactions with parasitic weeds and symbiotic arbuscular mycorrhizal fungi. Despite the rapid progress in elucidating the SL biosynthetic pathway, the perce...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096652/ https://www.ncbi.nlm.nih.gov/pubmed/24336215 http://dx.doi.org/10.1038/nature12878 |
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author | Zhou, Feng Lin, Qibing Zhu, Lihong Ren, Yulong Zhou, Kunneng Shabek, Nitzan Wu, Fuqing Mao, Haibin Dong, Wei Gan, Lu Ma, Weiwei Gao, He Chen, Jun Yang, Chao Wang, Dan Tan, Junjie Zhang, Xin Guo, Xiuping Wang, Jiulin Jiang, Ling Liu, Xi Chen, Weiqi Chu, Jinfang Yan, Cunyu Ueno, Kotomi Ito, Shinsaku Asami, Tadao Cheng, Zhijun Wang, Jie Lei, Cailin Zhai, Huqu Wu, Chuanyin Wang, Haiyang Zheng, Ning Wan, Jianmin |
author_facet | Zhou, Feng Lin, Qibing Zhu, Lihong Ren, Yulong Zhou, Kunneng Shabek, Nitzan Wu, Fuqing Mao, Haibin Dong, Wei Gan, Lu Ma, Weiwei Gao, He Chen, Jun Yang, Chao Wang, Dan Tan, Junjie Zhang, Xin Guo, Xiuping Wang, Jiulin Jiang, Ling Liu, Xi Chen, Weiqi Chu, Jinfang Yan, Cunyu Ueno, Kotomi Ito, Shinsaku Asami, Tadao Cheng, Zhijun Wang, Jie Lei, Cailin Zhai, Huqu Wu, Chuanyin Wang, Haiyang Zheng, Ning Wan, Jianmin |
author_sort | Zhou, Feng |
collection | PubMed |
description | Strigolactones (SLs) are a new class of carotenoid-derived phytohormones essential for developmental processes shaping plant architecture and interactions with parasitic weeds and symbiotic arbuscular mycorrhizal fungi. Despite the rapid progress in elucidating the SL biosynthetic pathway, the perception and signaling mechanisms of SL remain poorly understood. Here we show that DWARF53 (D53) acts as a repressor of SL signaling and SLs induce its degradation. We found that the rice d53 mutant, which produces an exaggerated number of tillers compared to wild type plants, is caused by a gain-of-function mutation and is insensitive to exogenous SL treatment. The D53 gene product shares predicted features with the class I Clp ATPase proteins and can form a complex with the α/β hydrolase protein DWARF14 (D14) and the F-box protein DWARF3 (D3), two previously identified signaling components potentially responsible for SL perception. We demonstrate that, in a D14- and D3-dependent manner, SLs induce D53 degradation by the proteasome and abrogate its activity in promoting axillary bud outgrowth. Our combined genetic and biochemical data reveal that D53 acts as a repressor of the SL signaling pathway, whose hormone-induced degradation represents a key molecular link between SL perception and responses. |
format | Online Article Text |
id | pubmed-4096652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-40966522014-07-15 D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signaling Zhou, Feng Lin, Qibing Zhu, Lihong Ren, Yulong Zhou, Kunneng Shabek, Nitzan Wu, Fuqing Mao, Haibin Dong, Wei Gan, Lu Ma, Weiwei Gao, He Chen, Jun Yang, Chao Wang, Dan Tan, Junjie Zhang, Xin Guo, Xiuping Wang, Jiulin Jiang, Ling Liu, Xi Chen, Weiqi Chu, Jinfang Yan, Cunyu Ueno, Kotomi Ito, Shinsaku Asami, Tadao Cheng, Zhijun Wang, Jie Lei, Cailin Zhai, Huqu Wu, Chuanyin Wang, Haiyang Zheng, Ning Wan, Jianmin Nature Article Strigolactones (SLs) are a new class of carotenoid-derived phytohormones essential for developmental processes shaping plant architecture and interactions with parasitic weeds and symbiotic arbuscular mycorrhizal fungi. Despite the rapid progress in elucidating the SL biosynthetic pathway, the perception and signaling mechanisms of SL remain poorly understood. Here we show that DWARF53 (D53) acts as a repressor of SL signaling and SLs induce its degradation. We found that the rice d53 mutant, which produces an exaggerated number of tillers compared to wild type plants, is caused by a gain-of-function mutation and is insensitive to exogenous SL treatment. The D53 gene product shares predicted features with the class I Clp ATPase proteins and can form a complex with the α/β hydrolase protein DWARF14 (D14) and the F-box protein DWARF3 (D3), two previously identified signaling components potentially responsible for SL perception. We demonstrate that, in a D14- and D3-dependent manner, SLs induce D53 degradation by the proteasome and abrogate its activity in promoting axillary bud outgrowth. Our combined genetic and biochemical data reveal that D53 acts as a repressor of the SL signaling pathway, whose hormone-induced degradation represents a key molecular link between SL perception and responses. 2013-12-11 2013-12-19 /pmc/articles/PMC4096652/ /pubmed/24336215 http://dx.doi.org/10.1038/nature12878 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Zhou, Feng Lin, Qibing Zhu, Lihong Ren, Yulong Zhou, Kunneng Shabek, Nitzan Wu, Fuqing Mao, Haibin Dong, Wei Gan, Lu Ma, Weiwei Gao, He Chen, Jun Yang, Chao Wang, Dan Tan, Junjie Zhang, Xin Guo, Xiuping Wang, Jiulin Jiang, Ling Liu, Xi Chen, Weiqi Chu, Jinfang Yan, Cunyu Ueno, Kotomi Ito, Shinsaku Asami, Tadao Cheng, Zhijun Wang, Jie Lei, Cailin Zhai, Huqu Wu, Chuanyin Wang, Haiyang Zheng, Ning Wan, Jianmin D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signaling |
title | D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signaling |
title_full | D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signaling |
title_fullStr | D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signaling |
title_full_unstemmed | D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signaling |
title_short | D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signaling |
title_sort | d14-scf(d3)-dependent degradation of d53 regulates strigolactone signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096652/ https://www.ncbi.nlm.nih.gov/pubmed/24336215 http://dx.doi.org/10.1038/nature12878 |
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