Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches

[Image: see text] Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonst...

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Autores principales: Izquierdo-Serra, Mercè, Gascón-Moya, Marta, Hirtz, Jan J., Pittolo, Silvia, Poskanzer, Kira E., Ferrer, Èric, Alibés, Ramon, Busqué, Félix, Yuste, Rafael, Hernando, Jordi, Gorostiza, Pau
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096865/
https://www.ncbi.nlm.nih.gov/pubmed/24857186
http://dx.doi.org/10.1021/ja5026326
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author Izquierdo-Serra, Mercè
Gascón-Moya, Marta
Hirtz, Jan J.
Pittolo, Silvia
Poskanzer, Kira E.
Ferrer, Èric
Alibés, Ramon
Busqué, Félix
Yuste, Rafael
Hernando, Jordi
Gorostiza, Pau
author_facet Izquierdo-Serra, Mercè
Gascón-Moya, Marta
Hirtz, Jan J.
Pittolo, Silvia
Poskanzer, Kira E.
Ferrer, Èric
Alibés, Ramon
Busqué, Félix
Yuste, Rafael
Hernando, Jordi
Gorostiza, Pau
author_sort Izquierdo-Serra, Mercè
collection PubMed
description [Image: see text] Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution.
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spelling pubmed-40968652014-12-18 Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches Izquierdo-Serra, Mercè Gascón-Moya, Marta Hirtz, Jan J. Pittolo, Silvia Poskanzer, Kira E. Ferrer, Èric Alibés, Ramon Busqué, Félix Yuste, Rafael Hernando, Jordi Gorostiza, Pau J Am Chem Soc [Image: see text] Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution. American Chemical Society 2014-05-23 2014-06-18 /pmc/articles/PMC4096865/ /pubmed/24857186 http://dx.doi.org/10.1021/ja5026326 Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Izquierdo-Serra, Mercè
Gascón-Moya, Marta
Hirtz, Jan J.
Pittolo, Silvia
Poskanzer, Kira E.
Ferrer, Èric
Alibés, Ramon
Busqué, Félix
Yuste, Rafael
Hernando, Jordi
Gorostiza, Pau
Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches
title Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches
title_full Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches
title_fullStr Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches
title_full_unstemmed Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches
title_short Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches
title_sort two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096865/
https://www.ncbi.nlm.nih.gov/pubmed/24857186
http://dx.doi.org/10.1021/ja5026326
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