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Roles of CXCL5 on migration and invasion of liver cancer cells

Inflammatory factors play a vital role in the progression of liver cancer, although exact factors and related mechanisms still remain unclear. The present study aimed at screening inflammatory factors related to liver cancer metastasis and investigating the potential mechanism by which cancer cells...

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Autores principales: Xu, Xiaojing, Huang, Peixin, Yang, Biwei, Wang, Xiangdong, Xia, Jinglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097051/
https://www.ncbi.nlm.nih.gov/pubmed/25011526
http://dx.doi.org/10.1186/1479-5876-12-193
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author Xu, Xiaojing
Huang, Peixin
Yang, Biwei
Wang, Xiangdong
Xia, Jinglin
author_facet Xu, Xiaojing
Huang, Peixin
Yang, Biwei
Wang, Xiangdong
Xia, Jinglin
author_sort Xu, Xiaojing
collection PubMed
description Inflammatory factors play a vital role in the progression of liver cancer, although exact factors and related mechanisms still remain unclear. The present study aimed at screening inflammatory factors related to liver cancer metastasis and investigating the potential mechanism by which cancer cells are recruited. We screened and validated inflammatory factors by microarray and RT-PCR. Small interfering RNA (siRNA) and recombinant protein were used to assess CXCL5 effects on the movement of liver cancer cells (LCCs). Our screening microarray demonstrated over-expression of CXCL5 in LCCs with high metastatic potentials. CXCL5 increased LCCs migration and invasion, probably through autocrine and paracrine mechanisms. CXCL5-CXCR2 and ERK1/2 pathways could play critical roles in the regulation of LCCs migration. Our data indicates that LCCs per se may act as the producer and receptor of CXCL5 responsible for liver cancer migration and invasion.
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spelling pubmed-40970512014-07-16 Roles of CXCL5 on migration and invasion of liver cancer cells Xu, Xiaojing Huang, Peixin Yang, Biwei Wang, Xiangdong Xia, Jinglin J Transl Med Research Inflammatory factors play a vital role in the progression of liver cancer, although exact factors and related mechanisms still remain unclear. The present study aimed at screening inflammatory factors related to liver cancer metastasis and investigating the potential mechanism by which cancer cells are recruited. We screened and validated inflammatory factors by microarray and RT-PCR. Small interfering RNA (siRNA) and recombinant protein were used to assess CXCL5 effects on the movement of liver cancer cells (LCCs). Our screening microarray demonstrated over-expression of CXCL5 in LCCs with high metastatic potentials. CXCL5 increased LCCs migration and invasion, probably through autocrine and paracrine mechanisms. CXCL5-CXCR2 and ERK1/2 pathways could play critical roles in the regulation of LCCs migration. Our data indicates that LCCs per se may act as the producer and receptor of CXCL5 responsible for liver cancer migration and invasion. BioMed Central 2014-07-10 /pmc/articles/PMC4097051/ /pubmed/25011526 http://dx.doi.org/10.1186/1479-5876-12-193 Text en Copyright © 2014 Xu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xu, Xiaojing
Huang, Peixin
Yang, Biwei
Wang, Xiangdong
Xia, Jinglin
Roles of CXCL5 on migration and invasion of liver cancer cells
title Roles of CXCL5 on migration and invasion of liver cancer cells
title_full Roles of CXCL5 on migration and invasion of liver cancer cells
title_fullStr Roles of CXCL5 on migration and invasion of liver cancer cells
title_full_unstemmed Roles of CXCL5 on migration and invasion of liver cancer cells
title_short Roles of CXCL5 on migration and invasion of liver cancer cells
title_sort roles of cxcl5 on migration and invasion of liver cancer cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097051/
https://www.ncbi.nlm.nih.gov/pubmed/25011526
http://dx.doi.org/10.1186/1479-5876-12-193
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