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Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation

DNA methylation is a reversible epigenetic mark regulating genome stability and function in many eukaryotes. In Arabidopsis, active DNA demethylation depends on the function of the ROS1 subfamily of genes that encode 5-methylcytosine DNA glycosylases/lyases. ROS1-mediated DNA demethylation plays a c...

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Autores principales: Yamamuro, Chizuko, Miki, Daisuke, Zheng, Zhimin, Ma, Jun, Wang, Jing, Yang, Zhenbiao, Dong, Juan, Zhu, Jian-Kang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097119/
https://www.ncbi.nlm.nih.gov/pubmed/24898766
http://dx.doi.org/10.1038/ncomms5062
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author Yamamuro, Chizuko
Miki, Daisuke
Zheng, Zhimin
Ma, Jun
Wang, Jing
Yang, Zhenbiao
Dong, Juan
Zhu, Jian-Kang
author_facet Yamamuro, Chizuko
Miki, Daisuke
Zheng, Zhimin
Ma, Jun
Wang, Jing
Yang, Zhenbiao
Dong, Juan
Zhu, Jian-Kang
author_sort Yamamuro, Chizuko
collection PubMed
description DNA methylation is a reversible epigenetic mark regulating genome stability and function in many eukaryotes. In Arabidopsis, active DNA demethylation depends on the function of the ROS1 subfamily of genes that encode 5-methylcytosine DNA glycosylases/lyases. ROS1-mediated DNA demethylation plays a critical role in the regulation of transgenes, transposable elements and some endogenous genes, but there have been no reports of clear developmental phenotypes in ros1 mutant plants. Here we report that, in the ros1 mutant, the promoter region of the peptide ligand gene EPF2 is hypermethylated, which greatly reduces EPF2 expression and thereby leads to a phenotype of overproduction of stomatal lineage cells. EPF2 gene expression in ros1 is restored and the defective epidermal cell patterning is suppressed by mutations in genes in the RNA-directed DNA methylation pathway. Our results show that active DNA demethylation combats the activity of RNA-directed DNA methylation to influence the initiation of stomatal lineage cells.
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spelling pubmed-40971192014-12-05 Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation Yamamuro, Chizuko Miki, Daisuke Zheng, Zhimin Ma, Jun Wang, Jing Yang, Zhenbiao Dong, Juan Zhu, Jian-Kang Nat Commun Article DNA methylation is a reversible epigenetic mark regulating genome stability and function in many eukaryotes. In Arabidopsis, active DNA demethylation depends on the function of the ROS1 subfamily of genes that encode 5-methylcytosine DNA glycosylases/lyases. ROS1-mediated DNA demethylation plays a critical role in the regulation of transgenes, transposable elements and some endogenous genes, but there have been no reports of clear developmental phenotypes in ros1 mutant plants. Here we report that, in the ros1 mutant, the promoter region of the peptide ligand gene EPF2 is hypermethylated, which greatly reduces EPF2 expression and thereby leads to a phenotype of overproduction of stomatal lineage cells. EPF2 gene expression in ros1 is restored and the defective epidermal cell patterning is suppressed by mutations in genes in the RNA-directed DNA methylation pathway. Our results show that active DNA demethylation combats the activity of RNA-directed DNA methylation to influence the initiation of stomatal lineage cells. 2014-06-05 /pmc/articles/PMC4097119/ /pubmed/24898766 http://dx.doi.org/10.1038/ncomms5062 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Yamamuro, Chizuko
Miki, Daisuke
Zheng, Zhimin
Ma, Jun
Wang, Jing
Yang, Zhenbiao
Dong, Juan
Zhu, Jian-Kang
Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation
title Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation
title_full Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation
title_fullStr Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation
title_full_unstemmed Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation
title_short Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation
title_sort overproduction of stomatal lineage cells in arabidopsis mutants defective in active dna demethylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097119/
https://www.ncbi.nlm.nih.gov/pubmed/24898766
http://dx.doi.org/10.1038/ncomms5062
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