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Effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans

The β(2)‐adrenergic system is an important regulator of human adipose tissue lipolysis. Polymorphisms that result in amino acid substitutions in the β(2)‐adrenergic receptor have been reported to alter lipolysis. We hypothesized that variations in the amino acid at position 16 of the β(2)‐adrenergic...

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Main Authors: Du, Shichun, Joyner, Michael J., Curry, Timothy B., Eisenach, John H., Johnson, Christopher P., Schrage, William G., Jensen, Michael D.
Format: Online Article Text
Language:English
Published: Wiley Periodicals, Inc. 2014
Subjects:
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4098745/
https://www.ncbi.nlm.nih.gov/pubmed/24844639
http://dx.doi.org/10.14814/phy2.12017
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author Du, Shichun
Joyner, Michael J.
Curry, Timothy B.
Eisenach, John H.
Johnson, Christopher P.
Schrage, William G.
Jensen, Michael D.
author_facet Du, Shichun
Joyner, Michael J.
Curry, Timothy B.
Eisenach, John H.
Johnson, Christopher P.
Schrage, William G.
Jensen, Michael D.
author_sort Du, Shichun
collection PubMed
description The β(2)‐adrenergic system is an important regulator of human adipose tissue lipolysis. Polymorphisms that result in amino acid substitutions in the β(2)‐adrenergic receptor have been reported to alter lipolysis. We hypothesized that variations in the amino acid at position 16 of the β(2)‐adrenergic receptor would result in different lipolytic responses to intravenous epinephrine and exercise. 17 volunteers homozygous for glycine at position 16 (Gly/Gly, nine female) and 16 volunteers homozygous for arginine at position 16 (Arg/Arg, eight female) of the β(2)‐adrenergic receptor participated in this study. On one study day participants received infusions of epinephrine at submaximal (5 ng kg(−1) min(−1)) and maximal (40 ng kg(−1) min(−1)) lipolytic doses. The other study day volunteers bicycled for 90 min at 50–60% of maximum oxygen consumption (VO(2)max). [9,10‐(3)H] Palmitate was infused both days to measure free fatty acid – palmitate kinetics. Oxygen consumption was measured using indirect calorimetry. Palmitate release rates in response to epinephrine and exercise were not different in the Gly/Gly and Arg/Arg participants. The only statistically significant difference we observed was a lesser ΔVO(2) in Arg/Arg volunteers in response to the submaximal epinephrine infusion. The polymorphisms resulting in Arg/Arg and Gly/Gly at position 16 of the β(2)‐adrenergic receptor do not result in clinically meaningful differences in lipolysis responses to epinephrine or submaximal exercise.
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spelling pubmed-40987452014-08-06 Effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans Du, Shichun Joyner, Michael J. Curry, Timothy B. Eisenach, John H. Johnson, Christopher P. Schrage, William G. Jensen, Michael D. Physiol Rep Original Research The β(2)‐adrenergic system is an important regulator of human adipose tissue lipolysis. Polymorphisms that result in amino acid substitutions in the β(2)‐adrenergic receptor have been reported to alter lipolysis. We hypothesized that variations in the amino acid at position 16 of the β(2)‐adrenergic receptor would result in different lipolytic responses to intravenous epinephrine and exercise. 17 volunteers homozygous for glycine at position 16 (Gly/Gly, nine female) and 16 volunteers homozygous for arginine at position 16 (Arg/Arg, eight female) of the β(2)‐adrenergic receptor participated in this study. On one study day participants received infusions of epinephrine at submaximal (5 ng kg(−1) min(−1)) and maximal (40 ng kg(−1) min(−1)) lipolytic doses. The other study day volunteers bicycled for 90 min at 50–60% of maximum oxygen consumption (VO(2)max). [9,10‐(3)H] Palmitate was infused both days to measure free fatty acid – palmitate kinetics. Oxygen consumption was measured using indirect calorimetry. Palmitate release rates in response to epinephrine and exercise were not different in the Gly/Gly and Arg/Arg participants. The only statistically significant difference we observed was a lesser ΔVO(2) in Arg/Arg volunteers in response to the submaximal epinephrine infusion. The polymorphisms resulting in Arg/Arg and Gly/Gly at position 16 of the β(2)‐adrenergic receptor do not result in clinically meaningful differences in lipolysis responses to epinephrine or submaximal exercise. Wiley Periodicals, Inc. 2014-05-20 /pmc/articles/PMC4098745/ /pubmed/24844639 http://dx.doi.org/10.14814/phy2.12017 Text en © 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Du, Shichun
Joyner, Michael J.
Curry, Timothy B.
Eisenach, John H.
Johnson, Christopher P.
Schrage, William G.
Jensen, Michael D.
Effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans
title Effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans
title_full Effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans
title_fullStr Effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans
title_full_unstemmed Effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans
title_short Effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans
title_sort effect of β(2)‐adrenergic receptor polymorphisms on epinephrine and exercise‐stimulated lipolysis in humans
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4098745/
https://www.ncbi.nlm.nih.gov/pubmed/24844639
http://dx.doi.org/10.14814/phy2.12017
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