A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus

Introduction. The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. Method. This study provides a specific and sensitive step-by-step pr...

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Detalles Bibliográficos
Autores principales: Tseng, Chun-Hsien, Tsai, Hsiang-Jung, Chang, Chung-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4098948/
https://www.ncbi.nlm.nih.gov/pubmed/25057497
http://dx.doi.org/10.1155/2014/653056
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author Tseng, Chun-Hsien
Tsai, Hsiang-Jung
Chang, Chung-Ming
author_facet Tseng, Chun-Hsien
Tsai, Hsiang-Jung
Chang, Chung-Ming
author_sort Tseng, Chun-Hsien
collection PubMed
description Introduction. The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. Method. This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. Result and Conclusion. This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.
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spelling pubmed-40989482014-07-23 A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus Tseng, Chun-Hsien Tsai, Hsiang-Jung Chang, Chung-Ming Biomed Res Int Research Article Introduction. The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. Method. This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. Result and Conclusion. This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research. Hindawi Publishing Corporation 2014 2014-06-26 /pmc/articles/PMC4098948/ /pubmed/25057497 http://dx.doi.org/10.1155/2014/653056 Text en Copyright © 2014 Chun-Hsien Tseng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tseng, Chun-Hsien
Tsai, Hsiang-Jung
Chang, Chung-Ming
A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus
title A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus
title_full A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus
title_fullStr A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus
title_full_unstemmed A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus
title_short A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus
title_sort complete molecular diagnostic procedure for applications in surveillance and subtyping of avian influenza virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4098948/
https://www.ncbi.nlm.nih.gov/pubmed/25057497
http://dx.doi.org/10.1155/2014/653056
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