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Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis

Plants produce an immense variety of specialized metabolites, many of which are of high value as their bioactive properties make them useful as for instance pharmaceuticals. The compounds are often produced at low levels in the plant, and due to their complex structures, chemical synthesis may not b...

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Autores principales: Lassen, Lærke Münter, Nielsen, Agnieszka Zygadlo, Olsen, Carl Erik, Bialek, Wojciech, Jensen, Kenneth, Møller, Birger Lindberg, Jensen, Poul Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099078/
https://www.ncbi.nlm.nih.gov/pubmed/25025215
http://dx.doi.org/10.1371/journal.pone.0102184
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author Lassen, Lærke Münter
Nielsen, Agnieszka Zygadlo
Olsen, Carl Erik
Bialek, Wojciech
Jensen, Kenneth
Møller, Birger Lindberg
Jensen, Poul Erik
author_facet Lassen, Lærke Münter
Nielsen, Agnieszka Zygadlo
Olsen, Carl Erik
Bialek, Wojciech
Jensen, Kenneth
Møller, Birger Lindberg
Jensen, Poul Erik
author_sort Lassen, Lærke Münter
collection PubMed
description Plants produce an immense variety of specialized metabolites, many of which are of high value as their bioactive properties make them useful as for instance pharmaceuticals. The compounds are often produced at low levels in the plant, and due to their complex structures, chemical synthesis may not be feasible. Here, we take advantage of the reducing equivalents generated in photosynthesis in developing an approach for producing plant bioactive natural compounds in a photosynthetic microorganism by functionally coupling a biosynthetic enzyme to photosystem I. This enables driving of the enzymatic reactions with electrons extracted from the photosynthetic electron transport chain. As a proof of concept, we have genetically fused the soluble catalytic domain of the cytochrome P450 CYP79A1, originating from the endoplasmic reticulum membranes of Sorghum bicolor, to a photosystem I subunit in the cyanobacterium Synechococcus sp. PCC 7002, thereby targeting it to the thylakoids. The engineered enzyme showed light-driven activity both in vivo and in vitro, demonstrating the possibility to achieve light-driven biosynthesis of high-value plant specialized metabolites in cyanobacteria.
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spelling pubmed-40990782014-07-18 Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis Lassen, Lærke Münter Nielsen, Agnieszka Zygadlo Olsen, Carl Erik Bialek, Wojciech Jensen, Kenneth Møller, Birger Lindberg Jensen, Poul Erik PLoS One Research Article Plants produce an immense variety of specialized metabolites, many of which are of high value as their bioactive properties make them useful as for instance pharmaceuticals. The compounds are often produced at low levels in the plant, and due to their complex structures, chemical synthesis may not be feasible. Here, we take advantage of the reducing equivalents generated in photosynthesis in developing an approach for producing plant bioactive natural compounds in a photosynthetic microorganism by functionally coupling a biosynthetic enzyme to photosystem I. This enables driving of the enzymatic reactions with electrons extracted from the photosynthetic electron transport chain. As a proof of concept, we have genetically fused the soluble catalytic domain of the cytochrome P450 CYP79A1, originating from the endoplasmic reticulum membranes of Sorghum bicolor, to a photosystem I subunit in the cyanobacterium Synechococcus sp. PCC 7002, thereby targeting it to the thylakoids. The engineered enzyme showed light-driven activity both in vivo and in vitro, demonstrating the possibility to achieve light-driven biosynthesis of high-value plant specialized metabolites in cyanobacteria. Public Library of Science 2014-07-15 /pmc/articles/PMC4099078/ /pubmed/25025215 http://dx.doi.org/10.1371/journal.pone.0102184 Text en © 2014 Lassen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lassen, Lærke Münter
Nielsen, Agnieszka Zygadlo
Olsen, Carl Erik
Bialek, Wojciech
Jensen, Kenneth
Møller, Birger Lindberg
Jensen, Poul Erik
Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis
title Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis
title_full Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis
title_fullStr Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis
title_full_unstemmed Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis
title_short Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis
title_sort anchoring a plant cytochrome p450 via psam to the thylakoids in synechococcus sp. pcc 7002: evidence for light-driven biosynthesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099078/
https://www.ncbi.nlm.nih.gov/pubmed/25025215
http://dx.doi.org/10.1371/journal.pone.0102184
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