Cargando…

Histone deacetylase expression patterns in developing murine optic nerve

BACKGROUND: Histone deacetylases (HDACs) play important roles in glial cell development and in disease states within multiple regions of the central nervous system. However, little is known about HDAC expression or function within the optic nerve. As a first step in understanding the role of HDACs i...

Descripción completa

Detalles Bibliográficos
Autores principales: Tiwari, Sarika, Dharmarajan, Subramanian, Shivanna, Mahesh, Otteson, Deborah C, Belecky-Adams, Teri L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099093/
https://www.ncbi.nlm.nih.gov/pubmed/25011550
http://dx.doi.org/10.1186/1471-213X-14-30
_version_ 1782326430311383040
author Tiwari, Sarika
Dharmarajan, Subramanian
Shivanna, Mahesh
Otteson, Deborah C
Belecky-Adams, Teri L
author_facet Tiwari, Sarika
Dharmarajan, Subramanian
Shivanna, Mahesh
Otteson, Deborah C
Belecky-Adams, Teri L
author_sort Tiwari, Sarika
collection PubMed
description BACKGROUND: Histone deacetylases (HDACs) play important roles in glial cell development and in disease states within multiple regions of the central nervous system. However, little is known about HDAC expression or function within the optic nerve. As a first step in understanding the role of HDACs in optic nerve, this study examines the spatio-temporal expression patterns of methylated histone 3 (K9), acetylated histone 3 (K18), and HDACs 1–6 and 8–11 in the developing murine optic nerve head. RESULTS: Using RT-qPCR, western blot and immunofluorescence, three stages were analyzed: embryonic day 16 (E16), when astrocyte precursors are found in the optic stalk, postnatal day 5 (P5), when immature astrocytes and oligodendrocytes are found throughout the optic nerve, and P30, when optic nerve astrocytes and oligodendrocytes are mature. Acetylated and methylated histone H3 immunoreactivity was co-localized in the nuclei of most SOX2 positive glia within the optic nerve head and adjacent optic nerve at all developmental stages. HDACs 1–11 were expressed in the optic nerve glial cells at all three stages of optic nerve development in the mouse, but showed temporal differences in overall levels and subcellular localization. HDACs 1 and 2 were predominantly nuclear throughout optic nerve development and glial cell maturation. HDACs 3, 5, 6, 8, and 11 were predominantly cytoplasmic, but showed nuclear localization in at least one stage of optic nerve development. HDACs 4, 9 and10 were predominantly cytoplasmic, with little to no nuclear expression at any time during the developmental stages examined. CONCLUSIONS: Our results showing that HDACs 1, 2, 3, 5, 6, 8, and 11 were each localized to the nuclei of SOX2 positive glia at some stages of optic nerve development and maturation and extend previous reports of HDAC expression in the aging optic nerve. These HDACs are candidates for further research to understand how chromatin remodeling through acetylation, deacetylation and methylation contributes to glial development as well as their injury response.
format Online
Article
Text
id pubmed-4099093
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40990932014-07-16 Histone deacetylase expression patterns in developing murine optic nerve Tiwari, Sarika Dharmarajan, Subramanian Shivanna, Mahesh Otteson, Deborah C Belecky-Adams, Teri L BMC Dev Biol Research Article BACKGROUND: Histone deacetylases (HDACs) play important roles in glial cell development and in disease states within multiple regions of the central nervous system. However, little is known about HDAC expression or function within the optic nerve. As a first step in understanding the role of HDACs in optic nerve, this study examines the spatio-temporal expression patterns of methylated histone 3 (K9), acetylated histone 3 (K18), and HDACs 1–6 and 8–11 in the developing murine optic nerve head. RESULTS: Using RT-qPCR, western blot and immunofluorescence, three stages were analyzed: embryonic day 16 (E16), when astrocyte precursors are found in the optic stalk, postnatal day 5 (P5), when immature astrocytes and oligodendrocytes are found throughout the optic nerve, and P30, when optic nerve astrocytes and oligodendrocytes are mature. Acetylated and methylated histone H3 immunoreactivity was co-localized in the nuclei of most SOX2 positive glia within the optic nerve head and adjacent optic nerve at all developmental stages. HDACs 1–11 were expressed in the optic nerve glial cells at all three stages of optic nerve development in the mouse, but showed temporal differences in overall levels and subcellular localization. HDACs 1 and 2 were predominantly nuclear throughout optic nerve development and glial cell maturation. HDACs 3, 5, 6, 8, and 11 were predominantly cytoplasmic, but showed nuclear localization in at least one stage of optic nerve development. HDACs 4, 9 and10 were predominantly cytoplasmic, with little to no nuclear expression at any time during the developmental stages examined. CONCLUSIONS: Our results showing that HDACs 1, 2, 3, 5, 6, 8, and 11 were each localized to the nuclei of SOX2 positive glia at some stages of optic nerve development and maturation and extend previous reports of HDAC expression in the aging optic nerve. These HDACs are candidates for further research to understand how chromatin remodeling through acetylation, deacetylation and methylation contributes to glial development as well as their injury response. BioMed Central 2014-07-09 /pmc/articles/PMC4099093/ /pubmed/25011550 http://dx.doi.org/10.1186/1471-213X-14-30 Text en Copyright © 2014 Tiwari et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Tiwari, Sarika
Dharmarajan, Subramanian
Shivanna, Mahesh
Otteson, Deborah C
Belecky-Adams, Teri L
Histone deacetylase expression patterns in developing murine optic nerve
title Histone deacetylase expression patterns in developing murine optic nerve
title_full Histone deacetylase expression patterns in developing murine optic nerve
title_fullStr Histone deacetylase expression patterns in developing murine optic nerve
title_full_unstemmed Histone deacetylase expression patterns in developing murine optic nerve
title_short Histone deacetylase expression patterns in developing murine optic nerve
title_sort histone deacetylase expression patterns in developing murine optic nerve
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099093/
https://www.ncbi.nlm.nih.gov/pubmed/25011550
http://dx.doi.org/10.1186/1471-213X-14-30
work_keys_str_mv AT tiwarisarika histonedeacetylaseexpressionpatternsindevelopingmurineopticnerve
AT dharmarajansubramanian histonedeacetylaseexpressionpatternsindevelopingmurineopticnerve
AT shivannamahesh histonedeacetylaseexpressionpatternsindevelopingmurineopticnerve
AT ottesondeborahc histonedeacetylaseexpressionpatternsindevelopingmurineopticnerve
AT beleckyadamsteril histonedeacetylaseexpressionpatternsindevelopingmurineopticnerve