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Nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid

In order to enhance the in vivo codelivery efficiency of gambogic acid (GA) and all-trans retinoic acid (ATRA), our strategy was to entrap GA in the self-assembled nanoparticles based on amphiphilic hyaluronic acid (HA)-ATRA (HRA) conjugate. In this way, GA and ATRA were loaded simultaneously in a n...

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Autores principales: Yao, Jing, Li, Yuanke, Sun, Xiaojing, Dahmani, Fatima Zohra, Liu, Hongpan, Zhou, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099196/
https://www.ncbi.nlm.nih.gov/pubmed/25045262
http://dx.doi.org/10.2147/IJN.S62793
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author Yao, Jing
Li, Yuanke
Sun, Xiaojing
Dahmani, Fatima Zohra
Liu, Hongpan
Zhou, Jianping
author_facet Yao, Jing
Li, Yuanke
Sun, Xiaojing
Dahmani, Fatima Zohra
Liu, Hongpan
Zhou, Jianping
author_sort Yao, Jing
collection PubMed
description In order to enhance the in vivo codelivery efficiency of gambogic acid (GA) and all-trans retinoic acid (ATRA), our strategy was to entrap GA in the self-assembled nanoparticles based on amphiphilic hyaluronic acid (HA)-ATRA (HRA) conjugate. In this way, GA and ATRA were loaded simultaneously in a nanocarrier and codelivered into the tumor cell through HA receptor-mediated endocytosis. GA-loaded HRA nanoparticles (GA-HRA) were prepared by a dialysis method, and their physicochemical characteristics were investigated as well. GA-HRA exhibited a high drug loading capacity (31.1%), had a particle size in the range of 100–150 nm, and good biocompatibility. HRA nanoparticles were effectively internalized by MCF-7 cells and translocated into the nucleus in a time-dependent manner. The in vivo imaging analysis demonstrated that the fluorescent signals in the tumor were markedly increased with DiR-loaded nanoparticles after intravenous administration compared to free DiR solution, suggesting it has excellent tumor targeting properties. More importantly, GA-HRA exhibited excellent in vivo efficacy with dramatically reduced toxicity. In conclusion, with the assistance of HRA nanoparticles, GA and ATRA can successfully realize an effective combination chemotherapy as well as tumor-targeted delivery.
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spelling pubmed-40991962014-07-18 Nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid Yao, Jing Li, Yuanke Sun, Xiaojing Dahmani, Fatima Zohra Liu, Hongpan Zhou, Jianping Int J Nanomedicine Original Research In order to enhance the in vivo codelivery efficiency of gambogic acid (GA) and all-trans retinoic acid (ATRA), our strategy was to entrap GA in the self-assembled nanoparticles based on amphiphilic hyaluronic acid (HA)-ATRA (HRA) conjugate. In this way, GA and ATRA were loaded simultaneously in a nanocarrier and codelivered into the tumor cell through HA receptor-mediated endocytosis. GA-loaded HRA nanoparticles (GA-HRA) were prepared by a dialysis method, and their physicochemical characteristics were investigated as well. GA-HRA exhibited a high drug loading capacity (31.1%), had a particle size in the range of 100–150 nm, and good biocompatibility. HRA nanoparticles were effectively internalized by MCF-7 cells and translocated into the nucleus in a time-dependent manner. The in vivo imaging analysis demonstrated that the fluorescent signals in the tumor were markedly increased with DiR-loaded nanoparticles after intravenous administration compared to free DiR solution, suggesting it has excellent tumor targeting properties. More importantly, GA-HRA exhibited excellent in vivo efficacy with dramatically reduced toxicity. In conclusion, with the assistance of HRA nanoparticles, GA and ATRA can successfully realize an effective combination chemotherapy as well as tumor-targeted delivery. Dove Medical Press 2014-07-10 /pmc/articles/PMC4099196/ /pubmed/25045262 http://dx.doi.org/10.2147/IJN.S62793 Text en © 2014 Yao et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Yao, Jing
Li, Yuanke
Sun, Xiaojing
Dahmani, Fatima Zohra
Liu, Hongpan
Zhou, Jianping
Nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid
title Nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid
title_full Nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid
title_fullStr Nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid
title_full_unstemmed Nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid
title_short Nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid
title_sort nanoparticle delivery and combination therapy of gambogic acid and all-trans retinoic acid
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099196/
https://www.ncbi.nlm.nih.gov/pubmed/25045262
http://dx.doi.org/10.2147/IJN.S62793
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