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The Tumor-Suppressive MicroRNA-135b Targets c-Myc in Osteoscarcoma
Osteosarcoma is the most common primary tumor of the bone. It leads to many deaths because of its rapid proliferation and metastasis. Recent studies have shown that microRNAs are important gene regulators that are involved in various cancer-related processes. In this study, we found that miR-135b wa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099365/ https://www.ncbi.nlm.nih.gov/pubmed/25025684 http://dx.doi.org/10.1371/journal.pone.0102621 |
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author | Liu, Zheng Zhang, Guangwu Li, Jian Liu, Jiabang Lv, Pengfeng |
author_facet | Liu, Zheng Zhang, Guangwu Li, Jian Liu, Jiabang Lv, Pengfeng |
author_sort | Liu, Zheng |
collection | PubMed |
description | Osteosarcoma is the most common primary tumor of the bone. It leads to many deaths because of its rapid proliferation and metastasis. Recent studies have shown that microRNAs are important gene regulators that are involved in various cancer-related processes. In this study, we found that miR-135b was down-regulated in both osteoscarcoma patient tumor tissues and osteoscarcoma cell lines in comparison to paired adjacent non-tumor bone tissue. We observed that a lower level of miR-135b was associated with metastasis. The ectopic expression of miR-135b markedly suppressed osteoscarcoma cell proliferation, migration, and invasion. Conversely, the inhibition of miR-135b expression dramatically accelerated cell proliferation, migration, and invasion. The forced expression of miR-135b in osteosarcoma cells resulted in a significant reduction in the protein level of c-Myc and repressed the activity of a luciferase reporter that contained the 3′-untranslated region of the c-Myc mRNA. These effects were abolished by the mutation of the predicted miR-135b-binding site, which indicates that c-Myc may be a miR-135b target gene. Moreover, the ectopic expression of c-Myc partially reversed the inhibition of cell proliferation and invasion that was caused by miR-135b. These data therefore suggest that miR-135b may function as a tumor suppressor to regulate osteosarcoma cell proliferation and invasion through a mechanism that targets the c-Myc oncogene. These findings indicate that miR-135b may play a role in the pathogenesis of osteosarcoma. |
format | Online Article Text |
id | pubmed-4099365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40993652014-07-18 The Tumor-Suppressive MicroRNA-135b Targets c-Myc in Osteoscarcoma Liu, Zheng Zhang, Guangwu Li, Jian Liu, Jiabang Lv, Pengfeng PLoS One Research Article Osteosarcoma is the most common primary tumor of the bone. It leads to many deaths because of its rapid proliferation and metastasis. Recent studies have shown that microRNAs are important gene regulators that are involved in various cancer-related processes. In this study, we found that miR-135b was down-regulated in both osteoscarcoma patient tumor tissues and osteoscarcoma cell lines in comparison to paired adjacent non-tumor bone tissue. We observed that a lower level of miR-135b was associated with metastasis. The ectopic expression of miR-135b markedly suppressed osteoscarcoma cell proliferation, migration, and invasion. Conversely, the inhibition of miR-135b expression dramatically accelerated cell proliferation, migration, and invasion. The forced expression of miR-135b in osteosarcoma cells resulted in a significant reduction in the protein level of c-Myc and repressed the activity of a luciferase reporter that contained the 3′-untranslated region of the c-Myc mRNA. These effects were abolished by the mutation of the predicted miR-135b-binding site, which indicates that c-Myc may be a miR-135b target gene. Moreover, the ectopic expression of c-Myc partially reversed the inhibition of cell proliferation and invasion that was caused by miR-135b. These data therefore suggest that miR-135b may function as a tumor suppressor to regulate osteosarcoma cell proliferation and invasion through a mechanism that targets the c-Myc oncogene. These findings indicate that miR-135b may play a role in the pathogenesis of osteosarcoma. Public Library of Science 2014-07-15 /pmc/articles/PMC4099365/ /pubmed/25025684 http://dx.doi.org/10.1371/journal.pone.0102621 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Zheng Zhang, Guangwu Li, Jian Liu, Jiabang Lv, Pengfeng The Tumor-Suppressive MicroRNA-135b Targets c-Myc in Osteoscarcoma |
title | The Tumor-Suppressive MicroRNA-135b Targets c-Myc in Osteoscarcoma |
title_full | The Tumor-Suppressive MicroRNA-135b Targets c-Myc in Osteoscarcoma |
title_fullStr | The Tumor-Suppressive MicroRNA-135b Targets c-Myc in Osteoscarcoma |
title_full_unstemmed | The Tumor-Suppressive MicroRNA-135b Targets c-Myc in Osteoscarcoma |
title_short | The Tumor-Suppressive MicroRNA-135b Targets c-Myc in Osteoscarcoma |
title_sort | tumor-suppressive microrna-135b targets c-myc in osteoscarcoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099365/ https://www.ncbi.nlm.nih.gov/pubmed/25025684 http://dx.doi.org/10.1371/journal.pone.0102621 |
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