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MiR-195 affects cell migration and cell proliferation by down-regulating DIEXF in Hirschsprung’s Disease
BACKGROUND: Hirschsprung’s disease (HSCR) is the most common congenital gut motility disorder. We aimed to investigate the roles of miR-195 in the pathogenesis of HSCR. METHODS: In this study, we measured the expression levels of miRNA, mRNA, and protein in colon tissues from 78 patients with HSCR a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099404/ https://www.ncbi.nlm.nih.gov/pubmed/25007945 http://dx.doi.org/10.1186/1471-230X-14-123 |
Sumario: | BACKGROUND: Hirschsprung’s disease (HSCR) is the most common congenital gut motility disorder. We aimed to investigate the roles of miR-195 in the pathogenesis of HSCR. METHODS: In this study, we measured the expression levels of miRNA, mRNA, and protein in colon tissues from 78 patients with HSCR and 66 controls without HSCR. Transwell, Cell Counting Kit-8 (CCK-8) and flow cytometry assay were employed to detect the function role of miR-195 in vitro. RESULTS: Our results showed that expression levels of miR-195 from patients with HSCR were significantly higher than control group; along with aberrant lower expression levels of digestive-organ expansion factor (DIEXF) were tested. Increased level of miR-195 could suppress the level of DIEXF in cell, which induced the impairment of cell migration and proliferation. CONCLUSIONS: Aberrant expression of miR-195 may involved in the pathogenesis of HSCR by down-regulated the level of DIEXF. |
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