Cargando…

The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin

BACKGROUND: The absence of horns, called polled phenotype, is the favored trait in modern cattle husbandry. To date, polled cattle are obtained primarily by dehorning calves. Dehorning is a practice that raises animal welfare issues, which can be addressed by selecting for genetically hornless cattl...

Descripción completa

Detalles Bibliográficos
Autores principales: Rothammer, Sophie, Capitan, Aurélien, Mullaart, Erik, Seichter, Doris, Russ, Ingolf, Medugorac, Ivica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099407/
https://www.ncbi.nlm.nih.gov/pubmed/24993890
http://dx.doi.org/10.1186/1297-9686-46-44
_version_ 1782326484789100544
author Rothammer, Sophie
Capitan, Aurélien
Mullaart, Erik
Seichter, Doris
Russ, Ingolf
Medugorac, Ivica
author_facet Rothammer, Sophie
Capitan, Aurélien
Mullaart, Erik
Seichter, Doris
Russ, Ingolf
Medugorac, Ivica
author_sort Rothammer, Sophie
collection PubMed
description BACKGROUND: The absence of horns, called polled phenotype, is the favored trait in modern cattle husbandry. To date, polled cattle are obtained primarily by dehorning calves. Dehorning is a practice that raises animal welfare issues, which can be addressed by selecting for genetically hornless cattle. In the past 20 years, there have been many studies worldwide to identify unique genetic markers in complete association with the polled trait in cattle and recently, two different alleles at the POLLED locus, both resulting in the absence of horns, were reported: (1) the Celtic allele, which is responsible for the polled phenotype in most breeds and for which a single candidate mutation was detected and (2) the Friesian allele, which is responsible for the polled phenotype predominantly in the Holstein-Friesian breed and in a few other breeds, but for which five candidate mutations were identified in a 260-kb haplotype. Further studies based on genome-wide sequencing and high-density SNP (single nucleotide polymorphism) genotyping confirmed the existence of the Celtic and Friesian variants and narrowed down the causal Friesian haplotype to an interval of 145 kb. RESULTS: Almost 6000 animals were genetically tested for the polled trait and we detected a recombinant animal which enabled us to reduce the Friesian POLLED haplotype to a single causal mutation, namely a 80-kb duplication. Moreover, our results clearly disagree with the recently reported perfect co-segregation of the POLLED mutation and a SNP at position 1 390 292 bp on bovine chromosome 1 in the Holstein-Friesian population. CONCLUSION: We conclude that the 80-kb duplication, as the only remaining variant within the shortened Friesian haplotype, represents the most likely causal mutation for the polled phenotype of Friesian origin.
format Online
Article
Text
id pubmed-4099407
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40994072014-07-17 The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin Rothammer, Sophie Capitan, Aurélien Mullaart, Erik Seichter, Doris Russ, Ingolf Medugorac, Ivica Genet Sel Evol Research BACKGROUND: The absence of horns, called polled phenotype, is the favored trait in modern cattle husbandry. To date, polled cattle are obtained primarily by dehorning calves. Dehorning is a practice that raises animal welfare issues, which can be addressed by selecting for genetically hornless cattle. In the past 20 years, there have been many studies worldwide to identify unique genetic markers in complete association with the polled trait in cattle and recently, two different alleles at the POLLED locus, both resulting in the absence of horns, were reported: (1) the Celtic allele, which is responsible for the polled phenotype in most breeds and for which a single candidate mutation was detected and (2) the Friesian allele, which is responsible for the polled phenotype predominantly in the Holstein-Friesian breed and in a few other breeds, but for which five candidate mutations were identified in a 260-kb haplotype. Further studies based on genome-wide sequencing and high-density SNP (single nucleotide polymorphism) genotyping confirmed the existence of the Celtic and Friesian variants and narrowed down the causal Friesian haplotype to an interval of 145 kb. RESULTS: Almost 6000 animals were genetically tested for the polled trait and we detected a recombinant animal which enabled us to reduce the Friesian POLLED haplotype to a single causal mutation, namely a 80-kb duplication. Moreover, our results clearly disagree with the recently reported perfect co-segregation of the POLLED mutation and a SNP at position 1 390 292 bp on bovine chromosome 1 in the Holstein-Friesian population. CONCLUSION: We conclude that the 80-kb duplication, as the only remaining variant within the shortened Friesian haplotype, represents the most likely causal mutation for the polled phenotype of Friesian origin. BioMed Central 2014-07-03 /pmc/articles/PMC4099407/ /pubmed/24993890 http://dx.doi.org/10.1186/1297-9686-46-44 Text en Copyright © 2014 Rothammer et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Rothammer, Sophie
Capitan, Aurélien
Mullaart, Erik
Seichter, Doris
Russ, Ingolf
Medugorac, Ivica
The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin
title The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin
title_full The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin
title_fullStr The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin
title_full_unstemmed The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin
title_short The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin
title_sort 80-kb dna duplication on bta1 is the only remaining candidate mutation for the polled phenotype of friesian origin
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099407/
https://www.ncbi.nlm.nih.gov/pubmed/24993890
http://dx.doi.org/10.1186/1297-9686-46-44
work_keys_str_mv AT rothammersophie the80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT capitanaurelien the80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT mullaarterik the80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT seichterdoris the80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT russingolf the80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT medugoracivica the80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT rothammersophie 80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT capitanaurelien 80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT mullaarterik 80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT seichterdoris 80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT russingolf 80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin
AT medugoracivica 80kbdnaduplicationonbta1istheonlyremainingcandidatemutationforthepolledphenotypeoffriesianorigin