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Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems

Since the establishment of embryonic stem (ES) cell lines, the combined use of gene targeting with homologous recombination has aided in elucidating the functions of various genes. However, the ES cell technique is inefficient and time-consuming. Recently, two new gene-targeting technologies have be...

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Detalles Bibliográficos
Autores principales:	Yasue, Akihiro, Mitsui, Silvia Naomi, Watanabe, Takahito, Sakuma, Tetsushi, Oyadomari, Seiichi, Yamamoto, Takashi, Noji, Sumihare, Mito, Taro, Tanaka, Eiji
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099983/ https://www.ncbi.nlm.nih.gov/pubmed/25027812 http://dx.doi.org/10.1038/srep05705

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