A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma

Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), clone...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Wen, Li, Shenghua, Zhang, Weijing, Sun, Jian, Ren, Guangda, Dong, Quanchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100105/
https://www.ncbi.nlm.nih.gov/pubmed/24879522
http://dx.doi.org/10.3390/ijms15069481
_version_ 1782326612505657344
author Wu, Wen
Li, Shenghua
Zhang, Weijing
Sun, Jian
Ren, Guangda
Dong, Quanchao
author_facet Wu, Wen
Li, Shenghua
Zhang, Weijing
Sun, Jian
Ren, Guangda
Dong, Quanchao
author_sort Wu, Wen
collection PubMed
description Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). Results: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. Conclusion: The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.
format Online
Article
Text
id pubmed-4100105
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-41001052014-07-16 A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma Wu, Wen Li, Shenghua Zhang, Weijing Sun, Jian Ren, Guangda Dong, Quanchao Int J Mol Sci Article Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). Results: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. Conclusion: The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies. MDPI 2014-05-28 /pmc/articles/PMC4100105/ /pubmed/24879522 http://dx.doi.org/10.3390/ijms15069481 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wu, Wen
Li, Shenghua
Zhang, Weijing
Sun, Jian
Ren, Guangda
Dong, Quanchao
A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma
title A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma
title_full A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma
title_fullStr A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma
title_full_unstemmed A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma
title_short A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma
title_sort novel vhh antibody targeting the b cell-activating factor for b-cell lymphoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100105/
https://www.ncbi.nlm.nih.gov/pubmed/24879522
http://dx.doi.org/10.3390/ijms15069481
work_keys_str_mv AT wuwen anovelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT lishenghua anovelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT zhangweijing anovelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT sunjian anovelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT renguangda anovelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT dongquanchao anovelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT wuwen novelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT lishenghua novelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT zhangweijing novelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT sunjian novelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT renguangda novelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma
AT dongquanchao novelvhhantibodytargetingthebcellactivatingfactorforbcelllymphoma

Ejemplares similares