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A Cautionary Note on the Use of Split-YFP/BiFC in Plant Protein-Protein Interaction Studies
Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions. BiFC is based on the restoration of fluorescen...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100113/ https://www.ncbi.nlm.nih.gov/pubmed/24886811 http://dx.doi.org/10.3390/ijms15069628 |