Cargando…

Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury

Idiopathic pulmonary fibrosis (IPF) is a progressing lethal disease with few clinically effective therapies. Corilagin is a tannin derivative which shows anti-inflammatory and antifibrotics properties and is potentiated in treating IPF. Here, we investigated the effect of corilagin on lung injury fo...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Zheng, Guo, Qiong-Ya, Zhang, Xiao-Ju, Li, Xiao, Li, Wen-Ting, Ma, Xi-Tao, Ma, Li-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100119/
https://www.ncbi.nlm.nih.gov/pubmed/24886817
http://dx.doi.org/10.3390/ijms15069762
_version_ 1782326615640899584
author Wang, Zheng
Guo, Qiong-Ya
Zhang, Xiao-Ju
Li, Xiao
Li, Wen-Ting
Ma, Xi-Tao
Ma, Li-Jun
author_facet Wang, Zheng
Guo, Qiong-Ya
Zhang, Xiao-Ju
Li, Xiao
Li, Wen-Ting
Ma, Xi-Tao
Ma, Li-Jun
author_sort Wang, Zheng
collection PubMed
description Idiopathic pulmonary fibrosis (IPF) is a progressing lethal disease with few clinically effective therapies. Corilagin is a tannin derivative which shows anti-inflammatory and antifibrotics properties and is potentiated in treating IPF. Here, we investigated the effect of corilagin on lung injury following bleomycin exposure in an animal model of pulmonary fibrosis. Corilagin abrogated bleomycin-induced lung fibrosis as assessed by H&E; Masson’s trichrome staining and lung hydroxyproline content in lung tissue. Corilagin reduced the number of apoptotic lung cells and prevented lung epithelial cells from membrane breakdown, effluence of lamellar bodies and thickening of the respiratory membrane. Bleomycin exposure induced expression of MDA, IKKα, phosphorylated IKKα (p-IKKα), NF-κB P65, TNF-α and IL-1β, and reduced I-κB expression in mice lung tissue or in BALF. These changes were reversed by high-dose corilagin (100 mg/kg i.p) more dramatically than by low dose (10 mg/kg i.p). Last, corilagin inhibits TGF-β1 production and α-SMA expression in lung tissue samples. Taken together, these findings confirmed that corilagin attenuates bleomycin-induced epithelial injury and fibrosis via inactivation of oxidative stress, proinflammatory cytokine release and NF-κB and TGF-β1 signaling. Corilagin may serve as a promising therapeutic agent for pulmonary fibrosis.
format Online
Article
Text
id pubmed-4100119
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-41001192014-07-16 Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury Wang, Zheng Guo, Qiong-Ya Zhang, Xiao-Ju Li, Xiao Li, Wen-Ting Ma, Xi-Tao Ma, Li-Jun Int J Mol Sci Article Idiopathic pulmonary fibrosis (IPF) is a progressing lethal disease with few clinically effective therapies. Corilagin is a tannin derivative which shows anti-inflammatory and antifibrotics properties and is potentiated in treating IPF. Here, we investigated the effect of corilagin on lung injury following bleomycin exposure in an animal model of pulmonary fibrosis. Corilagin abrogated bleomycin-induced lung fibrosis as assessed by H&E; Masson’s trichrome staining and lung hydroxyproline content in lung tissue. Corilagin reduced the number of apoptotic lung cells and prevented lung epithelial cells from membrane breakdown, effluence of lamellar bodies and thickening of the respiratory membrane. Bleomycin exposure induced expression of MDA, IKKα, phosphorylated IKKα (p-IKKα), NF-κB P65, TNF-α and IL-1β, and reduced I-κB expression in mice lung tissue or in BALF. These changes were reversed by high-dose corilagin (100 mg/kg i.p) more dramatically than by low dose (10 mg/kg i.p). Last, corilagin inhibits TGF-β1 production and α-SMA expression in lung tissue samples. Taken together, these findings confirmed that corilagin attenuates bleomycin-induced epithelial injury and fibrosis via inactivation of oxidative stress, proinflammatory cytokine release and NF-κB and TGF-β1 signaling. Corilagin may serve as a promising therapeutic agent for pulmonary fibrosis. MDPI 2014-05-30 /pmc/articles/PMC4100119/ /pubmed/24886817 http://dx.doi.org/10.3390/ijms15069762 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Zheng
Guo, Qiong-Ya
Zhang, Xiao-Ju
Li, Xiao
Li, Wen-Ting
Ma, Xi-Tao
Ma, Li-Jun
Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury
title Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury
title_full Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury
title_fullStr Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury
title_full_unstemmed Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury
title_short Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury
title_sort corilagin attenuates aerosol bleomycin-induced experimental lung injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100119/
https://www.ncbi.nlm.nih.gov/pubmed/24886817
http://dx.doi.org/10.3390/ijms15069762
work_keys_str_mv AT wangzheng corilaginattenuatesaerosolbleomycininducedexperimentallunginjury
AT guoqiongya corilaginattenuatesaerosolbleomycininducedexperimentallunginjury
AT zhangxiaoju corilaginattenuatesaerosolbleomycininducedexperimentallunginjury
AT lixiao corilaginattenuatesaerosolbleomycininducedexperimentallunginjury
AT liwenting corilaginattenuatesaerosolbleomycininducedexperimentallunginjury
AT maxitao corilaginattenuatesaerosolbleomycininducedexperimentallunginjury
AT malijun corilaginattenuatesaerosolbleomycininducedexperimentallunginjury