Total Protein Extraction for Metaproteomics Analysis of Methane Producing Biofilm: The Effects of Detergents

Protein recovery is crucial for shotgun metaproteomics to study the in situ functionality of microbial populations from complex biofilms but still poorly addressed by far. To fill this knowledge gap, we systematically evaluated the sample preparation with extraction buffers comprising four detergent...

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Detalles Bibliográficos
Autores principales: Huang, Hung-Jen, Chen, Wei-Yu, Wu, Jer-Horng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100146/
https://www.ncbi.nlm.nih.gov/pubmed/24914765
http://dx.doi.org/10.3390/ijms150610169

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100146/
https://www.ncbi.nlm.nih.gov/pubmed/24914765
http://dx.doi.org/10.3390/ijms150610169

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