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Total Protein Extraction for Metaproteomics Analysis of Methane Producing Biofilm: The Effects of Detergents
Protein recovery is crucial for shotgun metaproteomics to study the in situ functionality of microbial populations from complex biofilms but still poorly addressed by far. To fill this knowledge gap, we systematically evaluated the sample preparation with extraction buffers comprising four detergent...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MDPI
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100146/ https://www.ncbi.nlm.nih.gov/pubmed/24914765 http://dx.doi.org/10.3390/ijms150610169 |