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Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits

To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacte...

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Autores principales: Xu, Chen, Ma, Xia, Chen, Shiwen, Tao, Meifeng, Yuan, Lutao, Jing, Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100185/
https://www.ncbi.nlm.nih.gov/pubmed/24937688
http://dx.doi.org/10.3390/ijms150610855
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author Xu, Chen
Ma, Xia
Chen, Shiwen
Tao, Meifeng
Yuan, Lutao
Jing, Yao
author_facet Xu, Chen
Ma, Xia
Chen, Shiwen
Tao, Meifeng
Yuan, Lutao
Jing, Yao
author_sort Xu, Chen
collection PubMed
description To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacter xylinum. The dural defects of the rabbit model were repaired with BC membranes. All surgeries were performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. All animals were humanely euthanized by intravenous injection of phenobarbitone, at each time point, after the operation. Then, the histocompatibility and inflammatory effects of BC were examined by histological examination, real-time fluorescent quantitative polymerase chain reaction (PCR) and Western Blot. BC membranes evenly covered the surface of brain without adhesion. There were seldom inflammatory cells surrounding the membrane during the early postoperative period. The expression of inflammatory cytokines IL-1β, IL-6 and TNF-α as well as iNOS and COX-2 were lower in the BC group compared to the control group at 7, 14 and 21 days after implantation. BC can repair dural defects in rabbit and has a decreased inflammatory response compared to traditional materials. However, the long-term effects need to be validated in larger animals.
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spelling pubmed-41001852014-07-16 Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits Xu, Chen Ma, Xia Chen, Shiwen Tao, Meifeng Yuan, Lutao Jing, Yao Int J Mol Sci Article To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacter xylinum. The dural defects of the rabbit model were repaired with BC membranes. All surgeries were performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. All animals were humanely euthanized by intravenous injection of phenobarbitone, at each time point, after the operation. Then, the histocompatibility and inflammatory effects of BC were examined by histological examination, real-time fluorescent quantitative polymerase chain reaction (PCR) and Western Blot. BC membranes evenly covered the surface of brain without adhesion. There were seldom inflammatory cells surrounding the membrane during the early postoperative period. The expression of inflammatory cytokines IL-1β, IL-6 and TNF-α as well as iNOS and COX-2 were lower in the BC group compared to the control group at 7, 14 and 21 days after implantation. BC can repair dural defects in rabbit and has a decreased inflammatory response compared to traditional materials. However, the long-term effects need to be validated in larger animals. MDPI 2014-06-16 /pmc/articles/PMC4100185/ /pubmed/24937688 http://dx.doi.org/10.3390/ijms150610855 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Xu, Chen
Ma, Xia
Chen, Shiwen
Tao, Meifeng
Yuan, Lutao
Jing, Yao
Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits
title Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits
title_full Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits
title_fullStr Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits
title_full_unstemmed Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits
title_short Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits
title_sort bacterial cellulose membranes used as artificial substitutes for dural defection in rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100185/
https://www.ncbi.nlm.nih.gov/pubmed/24937688
http://dx.doi.org/10.3390/ijms150610855
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