Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy

High-speed large-scale 3D imaging of neuronal activity poses a major challenge in neuroscience. Here, we demonstrate intrinsically simultaneous functional imaging of neuronal activity at single neuron resolution for an entire Caenorhabditis elegans as well as for the whole-brain of larval zebrafish....

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Autores principales: Prevedel, Robert, Yoon, Young-Gyu, Hoffmann, Maximilian, Pak, Nikita, Wetzstein, Gordon, Kato, Saul, Schrödel, Tina, Raskar, Ramesh, Zimmer, Manuel, Boyden, Edward S., Vaziri, Alipasha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100252/
https://www.ncbi.nlm.nih.gov/pubmed/24836920
http://dx.doi.org/10.1038/nmeth.2964
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author Prevedel, Robert
Yoon, Young-Gyu
Hoffmann, Maximilian
Pak, Nikita
Wetzstein, Gordon
Kato, Saul
Schrödel, Tina
Raskar, Ramesh
Zimmer, Manuel
Boyden, Edward S.
Vaziri, Alipasha
author_facet Prevedel, Robert
Yoon, Young-Gyu
Hoffmann, Maximilian
Pak, Nikita
Wetzstein, Gordon
Kato, Saul
Schrödel, Tina
Raskar, Ramesh
Zimmer, Manuel
Boyden, Edward S.
Vaziri, Alipasha
author_sort Prevedel, Robert
collection PubMed
description High-speed large-scale 3D imaging of neuronal activity poses a major challenge in neuroscience. Here, we demonstrate intrinsically simultaneous functional imaging of neuronal activity at single neuron resolution for an entire Caenorhabditis elegans as well as for the whole-brain of larval zebrafish. Our technique captures dynamics of spiking neurons in volumes of ~700 μm x 700 μm x 200 μm at 20 Hz and its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
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spelling pubmed-41002522015-01-01 Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy Prevedel, Robert Yoon, Young-Gyu Hoffmann, Maximilian Pak, Nikita Wetzstein, Gordon Kato, Saul Schrödel, Tina Raskar, Ramesh Zimmer, Manuel Boyden, Edward S. Vaziri, Alipasha Nat Methods Article High-speed large-scale 3D imaging of neuronal activity poses a major challenge in neuroscience. Here, we demonstrate intrinsically simultaneous functional imaging of neuronal activity at single neuron resolution for an entire Caenorhabditis elegans as well as for the whole-brain of larval zebrafish. Our technique captures dynamics of spiking neurons in volumes of ~700 μm x 700 μm x 200 μm at 20 Hz and its simplicity makes it an attractive tool for high-speed volumetric calcium imaging. 2014-05-18 2014-07 /pmc/articles/PMC4100252/ /pubmed/24836920 http://dx.doi.org/10.1038/nmeth.2964 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Prevedel, Robert
Yoon, Young-Gyu
Hoffmann, Maximilian
Pak, Nikita
Wetzstein, Gordon
Kato, Saul
Schrödel, Tina
Raskar, Ramesh
Zimmer, Manuel
Boyden, Edward S.
Vaziri, Alipasha
Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy
title Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy
title_full Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy
title_fullStr Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy
title_full_unstemmed Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy
title_short Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy
title_sort simultaneous whole-animal 3d-imaging of neuronal activity using light-field microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100252/
https://www.ncbi.nlm.nih.gov/pubmed/24836920
http://dx.doi.org/10.1038/nmeth.2964
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