Cargando…

A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

INTRODUCTION: The optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the abili...

Descripción completa

Detalles Bibliográficos
Autores principales: Gasparotto, Vinícius P O, Landim-Alvarenga, Fernanda C, Oliveira, Alexandre L R, Simões, Gustavo Ferreira, Lima-Neto, João F, Barraviera, Benedito, Ferreira, Rui S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100340/
https://www.ncbi.nlm.nih.gov/pubmed/24916098
http://dx.doi.org/10.1186/scrt467
_version_ 1782326654346985472
author Gasparotto, Vinícius P O
Landim-Alvarenga, Fernanda C
Oliveira, Alexandre L R
Simões, Gustavo Ferreira
Lima-Neto, João F
Barraviera, Benedito
Ferreira, Rui S
author_facet Gasparotto, Vinícius P O
Landim-Alvarenga, Fernanda C
Oliveira, Alexandre L R
Simões, Gustavo Ferreira
Lima-Neto, João F
Barraviera, Benedito
Ferreira, Rui S
author_sort Gasparotto, Vinícius P O
collection PubMed
description INTRODUCTION: The optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the ability of cells to affect and detect the biological microenvironment. METHODS: The characterization of CD34, CD44 and CD90 expression on mesenchymal stem cells was performed by flow cytometry. In vitro growth and cell viability were evaluated by light and electron microscopy. Differentiation into osteogenic, adipogenic and chondrogenic lineages was induced. RESULTS: The fibrin sealant did not affect cell adhesion, proliferation or differentiation and allowed the adherence and growth of mesenchymal stem cells on its surface. Hoechst 33342 and propidium iodide staining demonstrated the viability of mesenchymal stem cells in contact with the fibrin sealant and the ability of the biomaterial to maintain cell survival. CONCLUSIONS: The new fibrin sealant is a three-dimensional scaffolding candidate that is capable of maintaining cell survival without interfering with differentiation, and might also be useful in drug delivery. Fibrin sealant has a low production cost, does not transmit infectious diseases from human blood and has properties of a suitable scaffold for stem cells because it permits the preparation of differentiated scaffolds that are suitable for every need.
format Online
Article
Text
id pubmed-4100340
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-41003402014-07-17 A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells Gasparotto, Vinícius P O Landim-Alvarenga, Fernanda C Oliveira, Alexandre L R Simões, Gustavo Ferreira Lima-Neto, João F Barraviera, Benedito Ferreira, Rui S Stem Cell Res Ther Research INTRODUCTION: The optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the ability of cells to affect and detect the biological microenvironment. METHODS: The characterization of CD34, CD44 and CD90 expression on mesenchymal stem cells was performed by flow cytometry. In vitro growth and cell viability were evaluated by light and electron microscopy. Differentiation into osteogenic, adipogenic and chondrogenic lineages was induced. RESULTS: The fibrin sealant did not affect cell adhesion, proliferation or differentiation and allowed the adherence and growth of mesenchymal stem cells on its surface. Hoechst 33342 and propidium iodide staining demonstrated the viability of mesenchymal stem cells in contact with the fibrin sealant and the ability of the biomaterial to maintain cell survival. CONCLUSIONS: The new fibrin sealant is a three-dimensional scaffolding candidate that is capable of maintaining cell survival without interfering with differentiation, and might also be useful in drug delivery. Fibrin sealant has a low production cost, does not transmit infectious diseases from human blood and has properties of a suitable scaffold for stem cells because it permits the preparation of differentiated scaffolds that are suitable for every need. BioMed Central 2014-06-10 /pmc/articles/PMC4100340/ /pubmed/24916098 http://dx.doi.org/10.1186/scrt467 Text en Copyright © 2014 Gasparotto et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Gasparotto, Vinícius P O
Landim-Alvarenga, Fernanda C
Oliveira, Alexandre L R
Simões, Gustavo Ferreira
Lima-Neto, João F
Barraviera, Benedito
Ferreira, Rui S
A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells
title A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells
title_full A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells
title_fullStr A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells
title_full_unstemmed A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells
title_short A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells
title_sort new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100340/
https://www.ncbi.nlm.nih.gov/pubmed/24916098
http://dx.doi.org/10.1186/scrt467
work_keys_str_mv AT gasparottoviniciuspo anewfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT landimalvarengafernandac anewfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT oliveiraalexandrelr anewfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT simoesgustavoferreira anewfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT limanetojoaof anewfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT barravierabenedito anewfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT ferreiraruis anewfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT gasparottoviniciuspo newfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT landimalvarengafernandac newfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT oliveiraalexandrelr newfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT simoesgustavoferreira newfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT limanetojoaof newfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT barravierabenedito newfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells
AT ferreiraruis newfibrinsealantasathreedimensionalscaffoldcandidateformesenchymalstemcells