Cargando…

Repairing the Osteochondral Defect in Goat with the Tissue-Engineered Osteochondral Graft Preconstructed in a Double-Chamber Stirring Bioreactor

To investigate the reparative efficacy of tissue-engineered osteochondral (TEO) graft for repairing the osteochondral defect in goat, we designed a double-chamber stirring bioreactor to construct the bone and cartilage composites simultaneously in one β-TCP scaffold and observed the reparative effec...

Descripción completa

Detalles Bibliográficos
Autores principales: Pei, Yang, Fan, Jun-jun, Zhang, Xiao-qiang, Zhang, Zhi-yong, Yu, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100384/
https://www.ncbi.nlm.nih.gov/pubmed/25061604
http://dx.doi.org/10.1155/2014/219203
_version_ 1782326665429385216
author Pei, Yang
Fan, Jun-jun
Zhang, Xiao-qiang
Zhang, Zhi-yong
Yu, Min
author_facet Pei, Yang
Fan, Jun-jun
Zhang, Xiao-qiang
Zhang, Zhi-yong
Yu, Min
author_sort Pei, Yang
collection PubMed
description To investigate the reparative efficacy of tissue-engineered osteochondral (TEO) graft for repairing the osteochondral defect in goat, we designed a double-chamber stirring bioreactor to construct the bone and cartilage composites simultaneously in one β-TCP scaffold and observed the reparative effect in vivo. The osteochondral defects were created in goats and all the animals were divided into 3 groups randomly. In groups A, the defect was treated with the TEO which was cultured with mechanical stimulation of stir; in group B, the defect was treated with TEO which was cultured without mechanical stimulation of stir; in groups C, the defect was treated without TEO. At 12 weeks and 24 weeks after operation, the reparative effects in different groups were assessed and compared. The results indicated that the reparative effect of the TEO cultured in the bioreactor was better than the control group, and mechanical stimulation of stir could further improve the reparative effect. We provided a feasible and effective method to construct the TEO for treatment of osteochondral defect using autologous BMSCs and the double-chamber bioreactor.
format Online
Article
Text
id pubmed-4100384
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-41003842014-07-24 Repairing the Osteochondral Defect in Goat with the Tissue-Engineered Osteochondral Graft Preconstructed in a Double-Chamber Stirring Bioreactor Pei, Yang Fan, Jun-jun Zhang, Xiao-qiang Zhang, Zhi-yong Yu, Min Biomed Res Int Research Article To investigate the reparative efficacy of tissue-engineered osteochondral (TEO) graft for repairing the osteochondral defect in goat, we designed a double-chamber stirring bioreactor to construct the bone and cartilage composites simultaneously in one β-TCP scaffold and observed the reparative effect in vivo. The osteochondral defects were created in goats and all the animals were divided into 3 groups randomly. In groups A, the defect was treated with the TEO which was cultured with mechanical stimulation of stir; in group B, the defect was treated with TEO which was cultured without mechanical stimulation of stir; in groups C, the defect was treated without TEO. At 12 weeks and 24 weeks after operation, the reparative effects in different groups were assessed and compared. The results indicated that the reparative effect of the TEO cultured in the bioreactor was better than the control group, and mechanical stimulation of stir could further improve the reparative effect. We provided a feasible and effective method to construct the TEO for treatment of osteochondral defect using autologous BMSCs and the double-chamber bioreactor. Hindawi Publishing Corporation 2014 2014-07-02 /pmc/articles/PMC4100384/ /pubmed/25061604 http://dx.doi.org/10.1155/2014/219203 Text en Copyright © 2014 Yang Pei et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pei, Yang
Fan, Jun-jun
Zhang, Xiao-qiang
Zhang, Zhi-yong
Yu, Min
Repairing the Osteochondral Defect in Goat with the Tissue-Engineered Osteochondral Graft Preconstructed in a Double-Chamber Stirring Bioreactor
title Repairing the Osteochondral Defect in Goat with the Tissue-Engineered Osteochondral Graft Preconstructed in a Double-Chamber Stirring Bioreactor
title_full Repairing the Osteochondral Defect in Goat with the Tissue-Engineered Osteochondral Graft Preconstructed in a Double-Chamber Stirring Bioreactor
title_fullStr Repairing the Osteochondral Defect in Goat with the Tissue-Engineered Osteochondral Graft Preconstructed in a Double-Chamber Stirring Bioreactor
title_full_unstemmed Repairing the Osteochondral Defect in Goat with the Tissue-Engineered Osteochondral Graft Preconstructed in a Double-Chamber Stirring Bioreactor
title_short Repairing the Osteochondral Defect in Goat with the Tissue-Engineered Osteochondral Graft Preconstructed in a Double-Chamber Stirring Bioreactor
title_sort repairing the osteochondral defect in goat with the tissue-engineered osteochondral graft preconstructed in a double-chamber stirring bioreactor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100384/
https://www.ncbi.nlm.nih.gov/pubmed/25061604
http://dx.doi.org/10.1155/2014/219203
work_keys_str_mv AT peiyang repairingtheosteochondraldefectingoatwiththetissueengineeredosteochondralgraftpreconstructedinadoublechamberstirringbioreactor
AT fanjunjun repairingtheosteochondraldefectingoatwiththetissueengineeredosteochondralgraftpreconstructedinadoublechamberstirringbioreactor
AT zhangxiaoqiang repairingtheosteochondraldefectingoatwiththetissueengineeredosteochondralgraftpreconstructedinadoublechamberstirringbioreactor
AT zhangzhiyong repairingtheosteochondraldefectingoatwiththetissueengineeredosteochondralgraftpreconstructedinadoublechamberstirringbioreactor
AT yumin repairingtheosteochondraldefectingoatwiththetissueengineeredosteochondralgraftpreconstructedinadoublechamberstirringbioreactor