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Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function
Termites consume an estimated 3–7 billion tonnes of lignocellulose annually, a role in nature which is unique for a single order of invertebrates. Their food is digested with the help of microbial symbionts, a relationship that has been recognized for 200 years and actively researched for at least a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100516/ https://www.ncbi.nlm.nih.gov/pubmed/25009054 http://dx.doi.org/10.1098/rspb.2014.0990 |
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author | Tokuda, Gaku Tsuboi, Yuuri Kihara, Kumiko Saitou, Seikou Moriya, Sigeharu Lo, Nathan Kikuchi, Jun |
author_facet | Tokuda, Gaku Tsuboi, Yuuri Kihara, Kumiko Saitou, Seikou Moriya, Sigeharu Lo, Nathan Kikuchi, Jun |
author_sort | Tokuda, Gaku |
collection | PubMed |
description | Termites consume an estimated 3–7 billion tonnes of lignocellulose annually, a role in nature which is unique for a single order of invertebrates. Their food is digested with the help of microbial symbionts, a relationship that has been recognized for 200 years and actively researched for at least a century. Although DNA- and RNA-based approaches have greatly refined the details of the process and the identities of the participants, the allocation of roles in space and time remains unclear. To resolve this issue, a pioneer study is reported using metabolomics to chart the in situ catabolism of (13)C-cellulose fed to the dampwood species Hodotermopsis sjostedti. The results confirm that the secretion of endogenous cellulases by the host may be significant to the digestive process and indicate that a major contribution by hindgut bacteria is phosphorolysis of cellodextrins or cellobiose. This study provides evidence that essential amino acid acquisition by termites occurs following the lysis of microbial tissue obtained via proctodaeal trophallaxis. |
format | Online Article Text |
id | pubmed-4100516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-41005162014-08-22 Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function Tokuda, Gaku Tsuboi, Yuuri Kihara, Kumiko Saitou, Seikou Moriya, Sigeharu Lo, Nathan Kikuchi, Jun Proc Biol Sci Research Articles Termites consume an estimated 3–7 billion tonnes of lignocellulose annually, a role in nature which is unique for a single order of invertebrates. Their food is digested with the help of microbial symbionts, a relationship that has been recognized for 200 years and actively researched for at least a century. Although DNA- and RNA-based approaches have greatly refined the details of the process and the identities of the participants, the allocation of roles in space and time remains unclear. To resolve this issue, a pioneer study is reported using metabolomics to chart the in situ catabolism of (13)C-cellulose fed to the dampwood species Hodotermopsis sjostedti. The results confirm that the secretion of endogenous cellulases by the host may be significant to the digestive process and indicate that a major contribution by hindgut bacteria is phosphorolysis of cellodextrins or cellobiose. This study provides evidence that essential amino acid acquisition by termites occurs following the lysis of microbial tissue obtained via proctodaeal trophallaxis. The Royal Society 2014-08-22 /pmc/articles/PMC4100516/ /pubmed/25009054 http://dx.doi.org/10.1098/rspb.2014.0990 Text en http://creativecommons.org/licenses/by/3.0/ © 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Articles Tokuda, Gaku Tsuboi, Yuuri Kihara, Kumiko Saitou, Seikou Moriya, Sigeharu Lo, Nathan Kikuchi, Jun Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function |
title | Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function |
title_full | Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function |
title_fullStr | Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function |
title_full_unstemmed | Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function |
title_short | Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function |
title_sort | metabolomic profiling of (13)c-labelled cellulose digestion in a lower termite: insights into gut symbiont function |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100516/ https://www.ncbi.nlm.nih.gov/pubmed/25009054 http://dx.doi.org/10.1098/rspb.2014.0990 |
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