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Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function

Termites consume an estimated 3–7 billion tonnes of lignocellulose annually, a role in nature which is unique for a single order of invertebrates. Their food is digested with the help of microbial symbionts, a relationship that has been recognized for 200 years and actively researched for at least a...

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Autores principales: Tokuda, Gaku, Tsuboi, Yuuri, Kihara, Kumiko, Saitou, Seikou, Moriya, Sigeharu, Lo, Nathan, Kikuchi, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100516/
https://www.ncbi.nlm.nih.gov/pubmed/25009054
http://dx.doi.org/10.1098/rspb.2014.0990
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author Tokuda, Gaku
Tsuboi, Yuuri
Kihara, Kumiko
Saitou, Seikou
Moriya, Sigeharu
Lo, Nathan
Kikuchi, Jun
author_facet Tokuda, Gaku
Tsuboi, Yuuri
Kihara, Kumiko
Saitou, Seikou
Moriya, Sigeharu
Lo, Nathan
Kikuchi, Jun
author_sort Tokuda, Gaku
collection PubMed
description Termites consume an estimated 3–7 billion tonnes of lignocellulose annually, a role in nature which is unique for a single order of invertebrates. Their food is digested with the help of microbial symbionts, a relationship that has been recognized for 200 years and actively researched for at least a century. Although DNA- and RNA-based approaches have greatly refined the details of the process and the identities of the participants, the allocation of roles in space and time remains unclear. To resolve this issue, a pioneer study is reported using metabolomics to chart the in situ catabolism of (13)C-cellulose fed to the dampwood species Hodotermopsis sjostedti. The results confirm that the secretion of endogenous cellulases by the host may be significant to the digestive process and indicate that a major contribution by hindgut bacteria is phosphorolysis of cellodextrins or cellobiose. This study provides evidence that essential amino acid acquisition by termites occurs following the lysis of microbial tissue obtained via proctodaeal trophallaxis.
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spelling pubmed-41005162014-08-22 Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function Tokuda, Gaku Tsuboi, Yuuri Kihara, Kumiko Saitou, Seikou Moriya, Sigeharu Lo, Nathan Kikuchi, Jun Proc Biol Sci Research Articles Termites consume an estimated 3–7 billion tonnes of lignocellulose annually, a role in nature which is unique for a single order of invertebrates. Their food is digested with the help of microbial symbionts, a relationship that has been recognized for 200 years and actively researched for at least a century. Although DNA- and RNA-based approaches have greatly refined the details of the process and the identities of the participants, the allocation of roles in space and time remains unclear. To resolve this issue, a pioneer study is reported using metabolomics to chart the in situ catabolism of (13)C-cellulose fed to the dampwood species Hodotermopsis sjostedti. The results confirm that the secretion of endogenous cellulases by the host may be significant to the digestive process and indicate that a major contribution by hindgut bacteria is phosphorolysis of cellodextrins or cellobiose. This study provides evidence that essential amino acid acquisition by termites occurs following the lysis of microbial tissue obtained via proctodaeal trophallaxis. The Royal Society 2014-08-22 /pmc/articles/PMC4100516/ /pubmed/25009054 http://dx.doi.org/10.1098/rspb.2014.0990 Text en http://creativecommons.org/licenses/by/3.0/ © 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research Articles
Tokuda, Gaku
Tsuboi, Yuuri
Kihara, Kumiko
Saitou, Seikou
Moriya, Sigeharu
Lo, Nathan
Kikuchi, Jun
Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function
title Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function
title_full Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function
title_fullStr Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function
title_full_unstemmed Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function
title_short Metabolomic profiling of (13)C-labelled cellulose digestion in a lower termite: insights into gut symbiont function
title_sort metabolomic profiling of (13)c-labelled cellulose digestion in a lower termite: insights into gut symbiont function
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100516/
https://www.ncbi.nlm.nih.gov/pubmed/25009054
http://dx.doi.org/10.1098/rspb.2014.0990
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