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Age-related increase of VGF-expression in T lymphocytes

VGF is a protein expressed by neurons and processed into several peptides. It plays a role in energy homeostasis and promotes growth and survival. Recently, VGF mRNA was detected in peripheral leukocytes. Since it is known that aging is associated with a decrease in the development and function of n...

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Autores principales: Busse, Stefan, Steiner, Johann, Micheel, Justus, Dobrowolny, Henrik, Mawrin, Christian, Krause, Tim J., Adamaszek, Michael, Bogerts, Bernhard, Bommhardt, Ursula, Hartig, Roland, Busse, Mandy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100807/
https://www.ncbi.nlm.nih.gov/pubmed/25013207
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author Busse, Stefan
Steiner, Johann
Micheel, Justus
Dobrowolny, Henrik
Mawrin, Christian
Krause, Tim J.
Adamaszek, Michael
Bogerts, Bernhard
Bommhardt, Ursula
Hartig, Roland
Busse, Mandy
author_facet Busse, Stefan
Steiner, Johann
Micheel, Justus
Dobrowolny, Henrik
Mawrin, Christian
Krause, Tim J.
Adamaszek, Michael
Bogerts, Bernhard
Bommhardt, Ursula
Hartig, Roland
Busse, Mandy
author_sort Busse, Stefan
collection PubMed
description VGF is a protein expressed by neurons and processed into several peptides. It plays a role in energy homeostasis and promotes growth and survival. Recently, VGF mRNA was detected in peripheral leukocytes. Since it is known that aging is associated with a decrease in the development and function of neuronal as well as immune cells, we addressed the question whether a peripheral expression of VGF by CD3+ T cells and CD56+ NK cells is correlated with age. Therefore, the frequency of VGF+CD3+ and VGF+CD56+ cells was determined in mentally healthy volunteers aged between 22 and 88. We found an age-dependent increase in the number of VGF+CD3+ T cells that correlated with HbA1c and the body mass index (BMI). VGF-expression by NK cells was age-independent. Blockade of VGF reduced proliferation and secretion of cytokines such as IL-2, IL-17A, IL-1β, IL-10 and TNF by CD3+ T cells and PBMCs. Rapamycin-mediated T cell blockade significantly reduced the frequency of VGF-expressing T cells. We conclude that VGF contributes to survival and function of peripheral T cells. The age-dependent increase in VGF-expression could serve as mechanism that counterregulates the decrease in functionality of T lymphocytes.
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spelling pubmed-41008072014-07-21 Age-related increase of VGF-expression in T lymphocytes Busse, Stefan Steiner, Johann Micheel, Justus Dobrowolny, Henrik Mawrin, Christian Krause, Tim J. Adamaszek, Michael Bogerts, Bernhard Bommhardt, Ursula Hartig, Roland Busse, Mandy Aging (Albany NY) Research Paper VGF is a protein expressed by neurons and processed into several peptides. It plays a role in energy homeostasis and promotes growth and survival. Recently, VGF mRNA was detected in peripheral leukocytes. Since it is known that aging is associated with a decrease in the development and function of neuronal as well as immune cells, we addressed the question whether a peripheral expression of VGF by CD3+ T cells and CD56+ NK cells is correlated with age. Therefore, the frequency of VGF+CD3+ and VGF+CD56+ cells was determined in mentally healthy volunteers aged between 22 and 88. We found an age-dependent increase in the number of VGF+CD3+ T cells that correlated with HbA1c and the body mass index (BMI). VGF-expression by NK cells was age-independent. Blockade of VGF reduced proliferation and secretion of cytokines such as IL-2, IL-17A, IL-1β, IL-10 and TNF by CD3+ T cells and PBMCs. Rapamycin-mediated T cell blockade significantly reduced the frequency of VGF-expressing T cells. We conclude that VGF contributes to survival and function of peripheral T cells. The age-dependent increase in VGF-expression could serve as mechanism that counterregulates the decrease in functionality of T lymphocytes. Impact Journals LLC 2014-05-04 /pmc/articles/PMC4100807/ /pubmed/25013207 Text en Copyright: © 2014 Busse et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Paper
Busse, Stefan
Steiner, Johann
Micheel, Justus
Dobrowolny, Henrik
Mawrin, Christian
Krause, Tim J.
Adamaszek, Michael
Bogerts, Bernhard
Bommhardt, Ursula
Hartig, Roland
Busse, Mandy
Age-related increase of VGF-expression in T lymphocytes
title Age-related increase of VGF-expression in T lymphocytes
title_full Age-related increase of VGF-expression in T lymphocytes
title_fullStr Age-related increase of VGF-expression in T lymphocytes
title_full_unstemmed Age-related increase of VGF-expression in T lymphocytes
title_short Age-related increase of VGF-expression in T lymphocytes
title_sort age-related increase of vgf-expression in t lymphocytes
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100807/
https://www.ncbi.nlm.nih.gov/pubmed/25013207
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