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The age related markers lipofuscin and apoptosis show different genetic architecture by QTL mapping in short-lived Nothobranchius fish

Annual fish of the genus Nothobranchius show large variations in lifespan and expression of age-related phenotypes between closely related populations. We studied N. kadleci and its sister species N. furzeri GRZ strain, and found that N.kadleci is longer-lived than the N. furzeri. Lipofuscin and apo...

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Autores principales: Ng'oma, Enoch, Reichwald, Kathrin, Dorn, Alexander, Wittig, Michael, Balschun, Tobias, Franke, Andre, Platzer, Matthias, Cellerino, Allesandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100809/
https://www.ncbi.nlm.nih.gov/pubmed/25093339
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author Ng'oma, Enoch
Reichwald, Kathrin
Dorn, Alexander
Wittig, Michael
Balschun, Tobias
Franke, Andre
Platzer, Matthias
Cellerino, Allesandro
author_facet Ng'oma, Enoch
Reichwald, Kathrin
Dorn, Alexander
Wittig, Michael
Balschun, Tobias
Franke, Andre
Platzer, Matthias
Cellerino, Allesandro
author_sort Ng'oma, Enoch
collection PubMed
description Annual fish of the genus Nothobranchius show large variations in lifespan and expression of age-related phenotypes between closely related populations. We studied N. kadleci and its sister species N. furzeri GRZ strain, and found that N.kadleci is longer-lived than the N. furzeri. Lipofuscin and apoptosis measured in the liver increased with age in N. kadleci with different profiles: lipofuscin increased linearly, while apoptosis declined in the oldest animals. More lipofuscin (P < 0.001) and apoptosis (P < 0.001) was observed in N. furzeri than in N. kadleci at 16w age. Lipofuscin and apoptotic cells were then quantified in hybrids from the mating of N. furzeri to N. kadleci. F(1) individuals showed heterosis for lipofuscin but additive effects for apoptosis. These two age-related phenotypes were not correlated in F(2) hybrids. Quantitative trait loci analysis of 287 F(2) fish using 237 markers identified two QTL accounting for 10% of lipofuscin variance (P < 0.001) with overdominance effect. Apoptotic cells revealed three significant- and two suggestive QTL explaining 19% of variance (P < 0.001), showing additive and dominance effects, and two interacting loci. Our results show that lipofuscin and apoptosis are markers of different age-dependent biological processes controlled by different genetic mechanisms.
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spelling pubmed-41008092014-07-21 The age related markers lipofuscin and apoptosis show different genetic architecture by QTL mapping in short-lived Nothobranchius fish Ng'oma, Enoch Reichwald, Kathrin Dorn, Alexander Wittig, Michael Balschun, Tobias Franke, Andre Platzer, Matthias Cellerino, Allesandro Aging (Albany NY) Research Paper Annual fish of the genus Nothobranchius show large variations in lifespan and expression of age-related phenotypes between closely related populations. We studied N. kadleci and its sister species N. furzeri GRZ strain, and found that N.kadleci is longer-lived than the N. furzeri. Lipofuscin and apoptosis measured in the liver increased with age in N. kadleci with different profiles: lipofuscin increased linearly, while apoptosis declined in the oldest animals. More lipofuscin (P < 0.001) and apoptosis (P < 0.001) was observed in N. furzeri than in N. kadleci at 16w age. Lipofuscin and apoptotic cells were then quantified in hybrids from the mating of N. furzeri to N. kadleci. F(1) individuals showed heterosis for lipofuscin but additive effects for apoptosis. These two age-related phenotypes were not correlated in F(2) hybrids. Quantitative trait loci analysis of 287 F(2) fish using 237 markers identified two QTL accounting for 10% of lipofuscin variance (P < 0.001) with overdominance effect. Apoptotic cells revealed three significant- and two suggestive QTL explaining 19% of variance (P < 0.001), showing additive and dominance effects, and two interacting loci. Our results show that lipofuscin and apoptosis are markers of different age-dependent biological processes controlled by different genetic mechanisms. Impact Journals LLC 2014-05-12 /pmc/articles/PMC4100809/ /pubmed/25093339 Text en Copyright: © 2014 Ng'oma et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Paper
Ng'oma, Enoch
Reichwald, Kathrin
Dorn, Alexander
Wittig, Michael
Balschun, Tobias
Franke, Andre
Platzer, Matthias
Cellerino, Allesandro
The age related markers lipofuscin and apoptosis show different genetic architecture by QTL mapping in short-lived Nothobranchius fish
title The age related markers lipofuscin and apoptosis show different genetic architecture by QTL mapping in short-lived Nothobranchius fish
title_full The age related markers lipofuscin and apoptosis show different genetic architecture by QTL mapping in short-lived Nothobranchius fish
title_fullStr The age related markers lipofuscin and apoptosis show different genetic architecture by QTL mapping in short-lived Nothobranchius fish
title_full_unstemmed The age related markers lipofuscin and apoptosis show different genetic architecture by QTL mapping in short-lived Nothobranchius fish
title_short The age related markers lipofuscin and apoptosis show different genetic architecture by QTL mapping in short-lived Nothobranchius fish
title_sort age related markers lipofuscin and apoptosis show different genetic architecture by qtl mapping in short-lived nothobranchius fish
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100809/
https://www.ncbi.nlm.nih.gov/pubmed/25093339
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