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Interaction between ROS dependent DNA damage, mitochondria and p38 MAPK underlies senescence of human adult stem cells

Human endometrium-derived mesenchymal stem cells (hMESCs) enter the premature senescence under sublethal oxidative stress, however underlying mechanism remains unknown. Here, we showed that exogenous H(2)O(2) induces a rapid phosphorylation and co-localization of ATM, H2A.X, 53BP1 leading to DNA dam...

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Autores principales: Borodkina, Aleksandra, Shatrova, Alla, Abushik, Polina, Nikolsky, Nikolay, Burova, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100810/
https://www.ncbi.nlm.nih.gov/pubmed/24934860
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author Borodkina, Aleksandra
Shatrova, Alla
Abushik, Polina
Nikolsky, Nikolay
Burova, Elena
author_facet Borodkina, Aleksandra
Shatrova, Alla
Abushik, Polina
Nikolsky, Nikolay
Burova, Elena
author_sort Borodkina, Aleksandra
collection PubMed
description Human endometrium-derived mesenchymal stem cells (hMESCs) enter the premature senescence under sublethal oxidative stress, however underlying mechanism remains unknown. Here, we showed that exogenous H(2)O(2) induces a rapid phosphorylation and co-localization of ATM, H2A.X, 53BP1 leading to DNA damage response (DDR) activation. DDR was accompanied with nuclear translocation of p-p53 followed by up-regulation of p21Waf1 and the permanent hypophosphorylation of pRb. Additionally, the increased p38MAPK/MAPKAPK-2 activation persisted in H(2)O(2)-treated cells. We suggest that both p53/p21/pRb and p38MAPK/MAPKAPK-2 pathways are responsible for establishing an irreversible cell cycle arrest that is typical of senescence. The process of further stabilization of senescence required prolonged DDR signaling activation that was provided by the permanent ROS production which in turn was regulated by both p38MAPK and the increased functional mitochondria. To reverse senescence, the pharmacological inhibition of p38MAPK was performed. Cell treatment with SB203580 was sufficient to recover partially senescence phenotype, to block the ROS elevation, to decrease the mitochondrial function, and finally to rescue proliferation. Thus, suppression of the p38MAPK pathway resulted in a partial prevention of H(2)O(2)-induced senescence of hMESCs. The current study is the first to reveal the molecular mechanism of the premature senescence of hMESCs in response to oxidative stress.
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spelling pubmed-41008102014-07-21 Interaction between ROS dependent DNA damage, mitochondria and p38 MAPK underlies senescence of human adult stem cells Borodkina, Aleksandra Shatrova, Alla Abushik, Polina Nikolsky, Nikolay Burova, Elena Aging (Albany NY) Research Paper Human endometrium-derived mesenchymal stem cells (hMESCs) enter the premature senescence under sublethal oxidative stress, however underlying mechanism remains unknown. Here, we showed that exogenous H(2)O(2) induces a rapid phosphorylation and co-localization of ATM, H2A.X, 53BP1 leading to DNA damage response (DDR) activation. DDR was accompanied with nuclear translocation of p-p53 followed by up-regulation of p21Waf1 and the permanent hypophosphorylation of pRb. Additionally, the increased p38MAPK/MAPKAPK-2 activation persisted in H(2)O(2)-treated cells. We suggest that both p53/p21/pRb and p38MAPK/MAPKAPK-2 pathways are responsible for establishing an irreversible cell cycle arrest that is typical of senescence. The process of further stabilization of senescence required prolonged DDR signaling activation that was provided by the permanent ROS production which in turn was regulated by both p38MAPK and the increased functional mitochondria. To reverse senescence, the pharmacological inhibition of p38MAPK was performed. Cell treatment with SB203580 was sufficient to recover partially senescence phenotype, to block the ROS elevation, to decrease the mitochondrial function, and finally to rescue proliferation. Thus, suppression of the p38MAPK pathway resulted in a partial prevention of H(2)O(2)-induced senescence of hMESCs. The current study is the first to reveal the molecular mechanism of the premature senescence of hMESCs in response to oxidative stress. Impact Journals LLC 2014-06-12 /pmc/articles/PMC4100810/ /pubmed/24934860 Text en Copyright: © 2014 Borodkina et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Paper
Borodkina, Aleksandra
Shatrova, Alla
Abushik, Polina
Nikolsky, Nikolay
Burova, Elena
Interaction between ROS dependent DNA damage, mitochondria and p38 MAPK underlies senescence of human adult stem cells
title Interaction between ROS dependent DNA damage, mitochondria and p38 MAPK underlies senescence of human adult stem cells
title_full Interaction between ROS dependent DNA damage, mitochondria and p38 MAPK underlies senescence of human adult stem cells
title_fullStr Interaction between ROS dependent DNA damage, mitochondria and p38 MAPK underlies senescence of human adult stem cells
title_full_unstemmed Interaction between ROS dependent DNA damage, mitochondria and p38 MAPK underlies senescence of human adult stem cells
title_short Interaction between ROS dependent DNA damage, mitochondria and p38 MAPK underlies senescence of human adult stem cells
title_sort interaction between ros dependent dna damage, mitochondria and p38 mapk underlies senescence of human adult stem cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100810/
https://www.ncbi.nlm.nih.gov/pubmed/24934860
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