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Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome
Dahlia variabilis, with an exceptionally high diversity of floral forms and colors, is a popular flower amongst both commercial growers and hobbyists. Recently, some genetic controls of pigment patterns have been elucidated. These studies have been limited, however, by the lack of comprehensive tran...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101353/ https://www.ncbi.nlm.nih.gov/pubmed/25101098 http://dx.doi.org/10.3389/fpls.2014.00340 |
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author | Lehnert, Erik M. Walbot, Virginia |
author_facet | Lehnert, Erik M. Walbot, Virginia |
author_sort | Lehnert, Erik M. |
collection | PubMed |
description | Dahlia variabilis, with an exceptionally high diversity of floral forms and colors, is a popular flower amongst both commercial growers and hobbyists. Recently, some genetic controls of pigment patterns have been elucidated. These studies have been limited, however, by the lack of comprehensive transcriptomic resources for this species. Here we report the sequencing, assembly, and annotation of the transcriptome of the developing leaves, stems, and floral buds of D. variabilis. This resulted in 35,638 contigs, most of which seem to contain the complete coding sequence, and of which 20,881 could be successfully annotated by similarity to UniProt. Furthermore, we conducted a preliminary investigation to identify contigs with expression patterns consistent with tissue-specificity. These results will accelerate research into the genetic controls of pigmentation and floral form of D. variabilis. |
format | Online Article Text |
id | pubmed-4101353 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-41013532014-08-06 Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome Lehnert, Erik M. Walbot, Virginia Front Plant Sci Plant Science Dahlia variabilis, with an exceptionally high diversity of floral forms and colors, is a popular flower amongst both commercial growers and hobbyists. Recently, some genetic controls of pigment patterns have been elucidated. These studies have been limited, however, by the lack of comprehensive transcriptomic resources for this species. Here we report the sequencing, assembly, and annotation of the transcriptome of the developing leaves, stems, and floral buds of D. variabilis. This resulted in 35,638 contigs, most of which seem to contain the complete coding sequence, and of which 20,881 could be successfully annotated by similarity to UniProt. Furthermore, we conducted a preliminary investigation to identify contigs with expression patterns consistent with tissue-specificity. These results will accelerate research into the genetic controls of pigmentation and floral form of D. variabilis. Frontiers Media S.A. 2014-07-17 /pmc/articles/PMC4101353/ /pubmed/25101098 http://dx.doi.org/10.3389/fpls.2014.00340 Text en Copyright © 2014 Lehnert and Walbot. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Lehnert, Erik M. Walbot, Virginia Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome |
title | Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome |
title_full | Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome |
title_fullStr | Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome |
title_full_unstemmed | Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome |
title_short | Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome |
title_sort | sequencing and de novo assembly of a dahlia hybrid cultivar transcriptome |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101353/ https://www.ncbi.nlm.nih.gov/pubmed/25101098 http://dx.doi.org/10.3389/fpls.2014.00340 |
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