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Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome

Dahlia variabilis, with an exceptionally high diversity of floral forms and colors, is a popular flower amongst both commercial growers and hobbyists. Recently, some genetic controls of pigment patterns have been elucidated. These studies have been limited, however, by the lack of comprehensive tran...

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Autores principales: Lehnert, Erik M., Walbot, Virginia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101353/
https://www.ncbi.nlm.nih.gov/pubmed/25101098
http://dx.doi.org/10.3389/fpls.2014.00340
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author Lehnert, Erik M.
Walbot, Virginia
author_facet Lehnert, Erik M.
Walbot, Virginia
author_sort Lehnert, Erik M.
collection PubMed
description Dahlia variabilis, with an exceptionally high diversity of floral forms and colors, is a popular flower amongst both commercial growers and hobbyists. Recently, some genetic controls of pigment patterns have been elucidated. These studies have been limited, however, by the lack of comprehensive transcriptomic resources for this species. Here we report the sequencing, assembly, and annotation of the transcriptome of the developing leaves, stems, and floral buds of D. variabilis. This resulted in 35,638 contigs, most of which seem to contain the complete coding sequence, and of which 20,881 could be successfully annotated by similarity to UniProt. Furthermore, we conducted a preliminary investigation to identify contigs with expression patterns consistent with tissue-specificity. These results will accelerate research into the genetic controls of pigmentation and floral form of D. variabilis.
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spelling pubmed-41013532014-08-06 Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome Lehnert, Erik M. Walbot, Virginia Front Plant Sci Plant Science Dahlia variabilis, with an exceptionally high diversity of floral forms and colors, is a popular flower amongst both commercial growers and hobbyists. Recently, some genetic controls of pigment patterns have been elucidated. These studies have been limited, however, by the lack of comprehensive transcriptomic resources for this species. Here we report the sequencing, assembly, and annotation of the transcriptome of the developing leaves, stems, and floral buds of D. variabilis. This resulted in 35,638 contigs, most of which seem to contain the complete coding sequence, and of which 20,881 could be successfully annotated by similarity to UniProt. Furthermore, we conducted a preliminary investigation to identify contigs with expression patterns consistent with tissue-specificity. These results will accelerate research into the genetic controls of pigmentation and floral form of D. variabilis. Frontiers Media S.A. 2014-07-17 /pmc/articles/PMC4101353/ /pubmed/25101098 http://dx.doi.org/10.3389/fpls.2014.00340 Text en Copyright © 2014 Lehnert and Walbot. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lehnert, Erik M.
Walbot, Virginia
Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome
title Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome
title_full Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome
title_fullStr Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome
title_full_unstemmed Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome
title_short Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome
title_sort sequencing and de novo assembly of a dahlia hybrid cultivar transcriptome
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101353/
https://www.ncbi.nlm.nih.gov/pubmed/25101098
http://dx.doi.org/10.3389/fpls.2014.00340
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