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Quantitative Metabolomics and Instationary (13)C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris

Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary (13)C metabolic flux analysis (INST (13)C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of the production of a Rhizopus oryzae lipase (...

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Detalles Bibliográficos
Autores principales:	Jordà, Joel, Cueto Rojas, Hugo, Carnicer, Marc, Wahl, Aljoscha, Ferrer, Pau, Albiol, Joan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101507/ https://www.ncbi.nlm.nih.gov/pubmed/24957027 http://dx.doi.org/10.3390/metabo4020281

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