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Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice

The ferritin core is composed of fine nanoparticulate Fe(3+) oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe(3+) polyoxohydroxide (nanoFe(3+)). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk ru...

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Autores principales: Aslam, Mohamad F., Frazer, David M., Faria, Nuno, Bruggraber, Sylvaine F. A., Wilkins, Sarah J., Mirciov, Cornel, Powell, Jonathan J., Anderson, Greg J., Pereira, Dora I. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Federation of American Societies for Experimental Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101650/
https://www.ncbi.nlm.nih.gov/pubmed/24776745
http://dx.doi.org/10.1096/fj.14-251520
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author Aslam, Mohamad F.
Frazer, David M.
Faria, Nuno
Bruggraber, Sylvaine F. A.
Wilkins, Sarah J.
Mirciov, Cornel
Powell, Jonathan J.
Anderson, Greg J.
Pereira, Dora I. A.
author_facet Aslam, Mohamad F.
Frazer, David M.
Faria, Nuno
Bruggraber, Sylvaine F. A.
Wilkins, Sarah J.
Mirciov, Cornel
Powell, Jonathan J.
Anderson, Greg J.
Pereira, Dora I. A.
author_sort Aslam, Mohamad F.
collection PubMed
description The ferritin core is composed of fine nanoparticulate Fe(3+) oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe(3+) polyoxohydroxide (nanoFe(3+)). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe(2+) sulfate (FeSO(4)), nanoFe(3+), or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe(3+) was investigated using isolated duodenal loops. Our data show that FeSO(4) and nanoFe(3+) are equally bioavailable in WT mice, and at wk 8 the mean ± sem hemoglobin increase was 18 ± 7 g/L in the FeSO(4) group and 30 ± 5 g/L in the nanoFe(3+) group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe(3+) is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement.—Aslam, M. F., Frazer, D. M., Faria, N., Bruggraber, S. F. A., Wilkins, S. J., Mirciov, C., Powell, J. J., Anderson, G. J., Pereira, D. I. A. Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice.
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spelling pubmed-41016502014-08-19 Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice Aslam, Mohamad F. Frazer, David M. Faria, Nuno Bruggraber, Sylvaine F. A. Wilkins, Sarah J. Mirciov, Cornel Powell, Jonathan J. Anderson, Greg J. Pereira, Dora I. A. FASEB J Research Communications The ferritin core is composed of fine nanoparticulate Fe(3+) oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe(3+) polyoxohydroxide (nanoFe(3+)). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe(2+) sulfate (FeSO(4)), nanoFe(3+), or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe(3+) was investigated using isolated duodenal loops. Our data show that FeSO(4) and nanoFe(3+) are equally bioavailable in WT mice, and at wk 8 the mean ± sem hemoglobin increase was 18 ± 7 g/L in the FeSO(4) group and 30 ± 5 g/L in the nanoFe(3+) group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe(3+) is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement.—Aslam, M. F., Frazer, D. M., Faria, N., Bruggraber, S. F. A., Wilkins, S. J., Mirciov, C., Powell, J. J., Anderson, G. J., Pereira, D. I. A. Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice. Federation of American Societies for Experimental Biology 2014-08 /pmc/articles/PMC4101650/ /pubmed/24776745 http://dx.doi.org/10.1096/fj.14-251520 Text en © FASEB This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) (http://creativecommons.org/licenses/by/3.0/deed.en_US) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Communications
Aslam, Mohamad F.
Frazer, David M.
Faria, Nuno
Bruggraber, Sylvaine F. A.
Wilkins, Sarah J.
Mirciov, Cornel
Powell, Jonathan J.
Anderson, Greg J.
Pereira, Dora I. A.
Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
title Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
title_full Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
title_fullStr Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
title_full_unstemmed Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
title_short Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
title_sort ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101650/
https://www.ncbi.nlm.nih.gov/pubmed/24776745
http://dx.doi.org/10.1096/fj.14-251520
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