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RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer

BACKGROUND: RNA binding proteins (RBPs) play a fundamental role in posttranscriptional control of gene expression. Different RBPs have oncogenic or tumor-suppressive functions on human cancers. RNPC1 belongs to the RNA recognition motif (RRM) family of RBPs, which could regulate expression of divers...

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Autores principales: Xue, Jin-Qiu, Xia, Tian-Song, Liang, Xiu-Qing, Zhou, Wenbin, Cheng, Lin, Shi, Liang, Wang, Ying, Ding, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101826/
https://www.ncbi.nlm.nih.gov/pubmed/24884756
http://dx.doi.org/10.1186/1471-2407-14-322
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author Xue, Jin-Qiu
Xia, Tian-Song
Liang, Xiu-Qing
Zhou, Wenbin
Cheng, Lin
Shi, Liang
Wang, Ying
Ding, Qiang
author_facet Xue, Jin-Qiu
Xia, Tian-Song
Liang, Xiu-Qing
Zhou, Wenbin
Cheng, Lin
Shi, Liang
Wang, Ying
Ding, Qiang
author_sort Xue, Jin-Qiu
collection PubMed
description BACKGROUND: RNA binding proteins (RBPs) play a fundamental role in posttranscriptional control of gene expression. Different RBPs have oncogenic or tumor-suppressive functions on human cancers. RNPC1 belongs to the RNA recognition motif (RRM) family of RBPs, which could regulate expression of diverse targets by mRNA stability in human cancer cells. Several studies reported that RNPC1 played an important role in cancer, mostly acting as an oncogene or up-regulating in tumors. However, its role in human breast cancer remains unclear. METHODS: In the present study, we investigated the functional and mechanistic roles of RNPC1 in attenuating invasive signal including reverse epithelial-mesenchymal transition (EMT) to inhibit breast cancer cells aggressiveness in vitro. Moreover, RNPC1 suppress tumorigenicity in vivo. Further, we studied the expression of RNPC1 in breast cancer tissue and adjacent normal breast tissue by quantitative RT-PCR (qRT-PCR) and Western blot. RESULTS: We observed that RNPC1 expression was silenced in breast cancer cell lines compared to breast epithelial cells. More important, RNPC1 was frequently silenced in breast cancer tissue compared to adjacent normal breast tissue. Low RNPC1 mRNA expression was associated with higher clinical stages and mutp53, while low level of RNPC1 protein was associated with higher lymph node metastasis, mutp53 and lower progesterone receptor (PR). Functional assays showed ectopic expression of RNPC1 could inhibit breast tumor cell proliferation in vivo and in vitro through inducing cell cycle arrest, and further suppress tumor cell migration and invasion partly through repressing mutant p53 (mutp53) induced EMT. CONCLUSIONS: Overall, our findings indicated that RNPC1 had a potential function to play a tumor-suppressor role which may be a potential marker in the therapeutic and prognostic of breast cancer.
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spelling pubmed-41018262014-07-18 RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer Xue, Jin-Qiu Xia, Tian-Song Liang, Xiu-Qing Zhou, Wenbin Cheng, Lin Shi, Liang Wang, Ying Ding, Qiang BMC Cancer Research Article BACKGROUND: RNA binding proteins (RBPs) play a fundamental role in posttranscriptional control of gene expression. Different RBPs have oncogenic or tumor-suppressive functions on human cancers. RNPC1 belongs to the RNA recognition motif (RRM) family of RBPs, which could regulate expression of diverse targets by mRNA stability in human cancer cells. Several studies reported that RNPC1 played an important role in cancer, mostly acting as an oncogene or up-regulating in tumors. However, its role in human breast cancer remains unclear. METHODS: In the present study, we investigated the functional and mechanistic roles of RNPC1 in attenuating invasive signal including reverse epithelial-mesenchymal transition (EMT) to inhibit breast cancer cells aggressiveness in vitro. Moreover, RNPC1 suppress tumorigenicity in vivo. Further, we studied the expression of RNPC1 in breast cancer tissue and adjacent normal breast tissue by quantitative RT-PCR (qRT-PCR) and Western blot. RESULTS: We observed that RNPC1 expression was silenced in breast cancer cell lines compared to breast epithelial cells. More important, RNPC1 was frequently silenced in breast cancer tissue compared to adjacent normal breast tissue. Low RNPC1 mRNA expression was associated with higher clinical stages and mutp53, while low level of RNPC1 protein was associated with higher lymph node metastasis, mutp53 and lower progesterone receptor (PR). Functional assays showed ectopic expression of RNPC1 could inhibit breast tumor cell proliferation in vivo and in vitro through inducing cell cycle arrest, and further suppress tumor cell migration and invasion partly through repressing mutant p53 (mutp53) induced EMT. CONCLUSIONS: Overall, our findings indicated that RNPC1 had a potential function to play a tumor-suppressor role which may be a potential marker in the therapeutic and prognostic of breast cancer. BioMed Central 2014-05-07 /pmc/articles/PMC4101826/ /pubmed/24884756 http://dx.doi.org/10.1186/1471-2407-14-322 Text en Copyright © 2014 Xue et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Xue, Jin-Qiu
Xia, Tian-Song
Liang, Xiu-Qing
Zhou, Wenbin
Cheng, Lin
Shi, Liang
Wang, Ying
Ding, Qiang
RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer
title RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer
title_full RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer
title_fullStr RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer
title_full_unstemmed RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer
title_short RNA-binding protein RNPC1: acting as a tumor suppressor in breast cancer
title_sort rna-binding protein rnpc1: acting as a tumor suppressor in breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101826/
https://www.ncbi.nlm.nih.gov/pubmed/24884756
http://dx.doi.org/10.1186/1471-2407-14-322
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