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17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells
A significant proportion of the genes regulated by 17-beta-estradiol (E2) via estrogen receptor alpha (ERα) have roles in vesicle trafficking in breast cancer. Intracellular vesicle trafficking and extracellular vesicles have important roles in tumourigenesis. Here we report the discovery of giant (...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102791/ https://www.ncbi.nlm.nih.gov/pubmed/24931391 |
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author | Wright, Paul K Jones, Sarah Bowen Ardern, Nicholas Ward, Rebecca Clarke, Robert B Sotgia, Federica Lisanti, Michael P Landberg, Goran Lamb, Rebecca |
author_facet | Wright, Paul K Jones, Sarah Bowen Ardern, Nicholas Ward, Rebecca Clarke, Robert B Sotgia, Federica Lisanti, Michael P Landberg, Goran Lamb, Rebecca |
author_sort | Wright, Paul K |
collection | PubMed |
description | A significant proportion of the genes regulated by 17-beta-estradiol (E2) via estrogen receptor alpha (ERα) have roles in vesicle trafficking in breast cancer. Intracellular vesicle trafficking and extracellular vesicles have important roles in tumourigenesis. Here we report the discovery of giant (3-42μm) intracellular and extracellular vesicles (GVs) and the role of E2 on vesicle formation in breast cancer (BC) cell lines using three independent live cell imaging techniques. Large diameter vesicles, GVs were also identified in a patient-derived xenograft BC model, and in invasive breast carcinoma tissue. ERα-positive (MCF-7 and T47D) BC cell lines demonstrated a significant increase in GV formation after stimulation with E2 which was reversed by tamoxifen. ERα-negative (MDA-MB-231 and MDA-MB-468) BC cell lines produced GVs independently of E2 and tamoxifen. These results indicate the existence of both intracellular and extracellular vesicles with considerably larger dimensions than generally recognised with BC cells and suggest that the GVs are regulated by E2 via ERα in ERα-positive BC but by E2-independent mechanisms in ER-ve BC. |
format | Online Article Text |
id | pubmed-4102791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-41027912014-07-23 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells Wright, Paul K Jones, Sarah Bowen Ardern, Nicholas Ward, Rebecca Clarke, Robert B Sotgia, Federica Lisanti, Michael P Landberg, Goran Lamb, Rebecca Oncotarget Research Paper A significant proportion of the genes regulated by 17-beta-estradiol (E2) via estrogen receptor alpha (ERα) have roles in vesicle trafficking in breast cancer. Intracellular vesicle trafficking and extracellular vesicles have important roles in tumourigenesis. Here we report the discovery of giant (3-42μm) intracellular and extracellular vesicles (GVs) and the role of E2 on vesicle formation in breast cancer (BC) cell lines using three independent live cell imaging techniques. Large diameter vesicles, GVs were also identified in a patient-derived xenograft BC model, and in invasive breast carcinoma tissue. ERα-positive (MCF-7 and T47D) BC cell lines demonstrated a significant increase in GV formation after stimulation with E2 which was reversed by tamoxifen. ERα-negative (MDA-MB-231 and MDA-MB-468) BC cell lines produced GVs independently of E2 and tamoxifen. These results indicate the existence of both intracellular and extracellular vesicles with considerably larger dimensions than generally recognised with BC cells and suggest that the GVs are regulated by E2 via ERα in ERα-positive BC but by E2-independent mechanisms in ER-ve BC. Impact Journals LLC 2014-03-15 /pmc/articles/PMC4102791/ /pubmed/24931391 Text en Copyright: © 2014 Wright et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wright, Paul K Jones, Sarah Bowen Ardern, Nicholas Ward, Rebecca Clarke, Robert B Sotgia, Federica Lisanti, Michael P Landberg, Goran Lamb, Rebecca 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells |
title | 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells |
title_full | 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells |
title_fullStr | 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells |
title_full_unstemmed | 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells |
title_short | 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells |
title_sort | 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102791/ https://www.ncbi.nlm.nih.gov/pubmed/24931391 |
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