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Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus

Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be used as a helicase probe in B. subtilis and closely...

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Detalles Bibliográficos
Autores principales:	Green, Matthew, Gilhooly, Neville S., Abedeen, Shahriar, Scott, David J., Dillingham, Mark S., Soultanas, Panos
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Advanced Technology 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103019/ https://www.ncbi.nlm.nih.gov/pubmed/24953846 http://dx.doi.org/10.1016/j.bios.2014.06.011

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