Cargando…
Mitochondrial microsatellite markers for the Australian ectomycorrhizal fungus Laccaria sp. A (Hydnangiaceae)(1)
• Premise of the study: Microsatellite loci were developed for the ectomycorrhizal fungus Laccaria sp. A to investigate the population genetic structure of this fungal symbiont across its fragmented distribution in southeastern Australia. • Methods and Results: A partial genome sequence from an indi...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103105/ https://www.ncbi.nlm.nih.gov/pubmed/25202611 http://dx.doi.org/10.3732/apps.1300086 |
_version_ | 1782327112931213312 |
---|---|
author | Sheedy, Elizabeth M. Van de Wouw, Angela P. Howlett, Barbara J. May, Tom W. |
author_facet | Sheedy, Elizabeth M. Van de Wouw, Angela P. Howlett, Barbara J. May, Tom W. |
author_sort | Sheedy, Elizabeth M. |
collection | PubMed |
description | • Premise of the study: Microsatellite loci were developed for the ectomycorrhizal fungus Laccaria sp. A to investigate the population genetic structure of this fungal symbiont across its fragmented distribution in southeastern Australia. • Methods and Results: A partial genome sequence from an individual collection of Laccaria sp. A was obtained by 454 genome sequencing. Eight microsatellite markers were selected from 66 loci identified in the genome. The selected markers were highly polymorphic (4–19 alleles per locus, average 13 alleles) and amplified reproducibly from collections made across the distribution of this species. Five of these markers also amplified reproducibly in the sister species Laccaria sp. E (1). All eight of the selected microsatellite loci were from the mitochondrial genome. • Conclusions: The highly polymorphic markers described here will enable population structure of Laccaria sp. A to be determined, contributing to research on mycorrhizal fungi from a novel distribution. |
format | Online Article Text |
id | pubmed-4103105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41031052014-09-08 Mitochondrial microsatellite markers for the Australian ectomycorrhizal fungus Laccaria sp. A (Hydnangiaceae)(1) Sheedy, Elizabeth M. Van de Wouw, Angela P. Howlett, Barbara J. May, Tom W. Appl Plant Sci Primer Note • Premise of the study: Microsatellite loci were developed for the ectomycorrhizal fungus Laccaria sp. A to investigate the population genetic structure of this fungal symbiont across its fragmented distribution in southeastern Australia. • Methods and Results: A partial genome sequence from an individual collection of Laccaria sp. A was obtained by 454 genome sequencing. Eight microsatellite markers were selected from 66 loci identified in the genome. The selected markers were highly polymorphic (4–19 alleles per locus, average 13 alleles) and amplified reproducibly from collections made across the distribution of this species. Five of these markers also amplified reproducibly in the sister species Laccaria sp. E (1). All eight of the selected microsatellite loci were from the mitochondrial genome. • Conclusions: The highly polymorphic markers described here will enable population structure of Laccaria sp. A to be determined, contributing to research on mycorrhizal fungi from a novel distribution. Botanical Society of America 2014-02-11 /pmc/articles/PMC4103105/ /pubmed/25202611 http://dx.doi.org/10.3732/apps.1300086 Text en © 2014 Sheedy et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Sheedy, Elizabeth M. Van de Wouw, Angela P. Howlett, Barbara J. May, Tom W. Mitochondrial microsatellite markers for the Australian ectomycorrhizal fungus Laccaria sp. A (Hydnangiaceae)(1) |
title | Mitochondrial microsatellite markers for the Australian ectomycorrhizal fungus Laccaria sp. A (Hydnangiaceae)(1) |
title_full | Mitochondrial microsatellite markers for the Australian ectomycorrhizal fungus Laccaria sp. A (Hydnangiaceae)(1) |
title_fullStr | Mitochondrial microsatellite markers for the Australian ectomycorrhizal fungus Laccaria sp. A (Hydnangiaceae)(1) |
title_full_unstemmed | Mitochondrial microsatellite markers for the Australian ectomycorrhizal fungus Laccaria sp. A (Hydnangiaceae)(1) |
title_short | Mitochondrial microsatellite markers for the Australian ectomycorrhizal fungus Laccaria sp. A (Hydnangiaceae)(1) |
title_sort | mitochondrial microsatellite markers for the australian ectomycorrhizal fungus laccaria sp. a (hydnangiaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103105/ https://www.ncbi.nlm.nih.gov/pubmed/25202611 http://dx.doi.org/10.3732/apps.1300086 |
work_keys_str_mv | AT sheedyelizabethm mitochondrialmicrosatellitemarkersfortheaustralianectomycorrhizalfunguslaccariaspahydnangiaceae1 AT vandewouwangelap mitochondrialmicrosatellitemarkersfortheaustralianectomycorrhizalfunguslaccariaspahydnangiaceae1 AT howlettbarbaraj mitochondrialmicrosatellitemarkersfortheaustralianectomycorrhizalfunguslaccariaspahydnangiaceae1 AT maytomw mitochondrialmicrosatellitemarkersfortheaustralianectomycorrhizalfunguslaccariaspahydnangiaceae1 |