Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)(1)

• Premise of the study: Microsatellite markers were developed for the coastal shrub species Scaevola taccada to estimate the population genetic structure, which may reflect different seed dispersal patterns. • Methods and Results: Thirteen microsatellite primer sets were developed for S. taccada usi...

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Detalles Bibliográficos
Autores principales: Ando, Haruko, Emura, Naoko, Denda, Tetsuro, Nakahama, Naoyuki, Inoue-Murayama, Miho, Isagi, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2014
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103108/
https://www.ncbi.nlm.nih.gov/pubmed/25202622
http://dx.doi.org/10.3732/apps.1300094
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author Ando, Haruko
Emura, Naoko
Denda, Tetsuro
Nakahama, Naoyuki
Inoue-Murayama, Miho
Isagi, Yuji
author_facet Ando, Haruko
Emura, Naoko
Denda, Tetsuro
Nakahama, Naoyuki
Inoue-Murayama, Miho
Isagi, Yuji
author_sort Ando, Haruko
collection PubMed
description • Premise of the study: Microsatellite markers were developed for the coastal shrub species Scaevola taccada to estimate the population genetic structure, which may reflect different seed dispersal patterns. • Methods and Results: Thirteen microsatellite primer sets were developed for S. taccada using 454 pyrosequencing. The primer sets were tested on 64 individuals sampled from two populations in Japan. Fragments were amplified using the primers, with one to 10 alleles per locus, and the expected heterozygosity ranged from 0.00 to 0.85. • Conclusions: These results indicate the utility of markers in S. taccada for broad estimations of the population genetic structure of this species.
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spelling pubmed-41031082014-09-08 Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)(1) Ando, Haruko Emura, Naoko Denda, Tetsuro Nakahama, Naoyuki Inoue-Murayama, Miho Isagi, Yuji Appl Plant Sci Primer Note • Premise of the study: Microsatellite markers were developed for the coastal shrub species Scaevola taccada to estimate the population genetic structure, which may reflect different seed dispersal patterns. • Methods and Results: Thirteen microsatellite primer sets were developed for S. taccada using 454 pyrosequencing. The primer sets were tested on 64 individuals sampled from two populations in Japan. Fragments were amplified using the primers, with one to 10 alleles per locus, and the expected heterozygosity ranged from 0.00 to 0.85. • Conclusions: These results indicate the utility of markers in S. taccada for broad estimations of the population genetic structure of this species. Botanical Society of America 2014-04-18 /pmc/articles/PMC4103108/ /pubmed/25202622 http://dx.doi.org/10.3732/apps.1300094 Text en © 2014 Ando et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Ando, Haruko
Emura, Naoko
Denda, Tetsuro
Nakahama, Naoyuki
Inoue-Murayama, Miho
Isagi, Yuji
Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)(1)
title Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)(1)
title_full Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)(1)
title_fullStr Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)(1)
title_full_unstemmed Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)(1)
title_short Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)(1)
title_sort development of microsatellite markers for the coastal shrub scaevola taccada (goodeniaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103108/
https://www.ncbi.nlm.nih.gov/pubmed/25202622
http://dx.doi.org/10.3732/apps.1300094
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