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Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1)

• Premise of the study: We developed microsatellite markers for Fimbristylis sericea, a coastal herb found in sand dunes in Asia and Australia. • Methods and Results: Twelve microsatellite loci were isolated, and the genetic variability within and among local populations was characterized. The numbe...

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Autores principales: Yamauchi, Satoka, Ohsako, Takanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103114/
https://www.ncbi.nlm.nih.gov/pubmed/25202628
http://dx.doi.org/10.3732/apps.1400026
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author Yamauchi, Satoka
Ohsako, Takanori
author_facet Yamauchi, Satoka
Ohsako, Takanori
author_sort Yamauchi, Satoka
collection PubMed
description • Premise of the study: We developed microsatellite markers for Fimbristylis sericea, a coastal herb found in sand dunes in Asia and Australia. • Methods and Results: Twelve microsatellite loci were isolated, and the genetic variability within and among local populations was characterized. The number of alleles per locus was two to five with a mean of 3.5, total expected heterozygosity per locus was 0.069–0.645 with a mean of 0.336, and average expected heterozygosity within a population per locus was 0.051–0.230. Most of the loci deviated significantly from Hardy–Weinberg equilibrium. • Conclusions: All 12 microsatellite loci were polymorphic within and among populations. These loci could be useful genetic markers for population genetic studies of F. sericea populations.
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spelling pubmed-41031142014-09-08 Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1) Yamauchi, Satoka Ohsako, Takanori Appl Plant Sci Primer Note • Premise of the study: We developed microsatellite markers for Fimbristylis sericea, a coastal herb found in sand dunes in Asia and Australia. • Methods and Results: Twelve microsatellite loci were isolated, and the genetic variability within and among local populations was characterized. The number of alleles per locus was two to five with a mean of 3.5, total expected heterozygosity per locus was 0.069–0.645 with a mean of 0.336, and average expected heterozygosity within a population per locus was 0.051–0.230. Most of the loci deviated significantly from Hardy–Weinberg equilibrium. • Conclusions: All 12 microsatellite loci were polymorphic within and among populations. These loci could be useful genetic markers for population genetic studies of F. sericea populations. Botanical Society of America 2014-05-07 /pmc/articles/PMC4103114/ /pubmed/25202628 http://dx.doi.org/10.3732/apps.1400026 Text en © 2014 Yamauchi and Ohsako. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Yamauchi, Satoka
Ohsako, Takanori
Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1)
title Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1)
title_full Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1)
title_fullStr Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1)
title_full_unstemmed Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1)
title_short Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1)
title_sort isolation and characterization of microsatellite loci in fimbristylis sericea (cyperaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103114/
https://www.ncbi.nlm.nih.gov/pubmed/25202628
http://dx.doi.org/10.3732/apps.1400026
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