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Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1)
• Premise of the study: We developed microsatellite markers for Fimbristylis sericea, a coastal herb found in sand dunes in Asia and Australia. • Methods and Results: Twelve microsatellite loci were isolated, and the genetic variability within and among local populations was characterized. The numbe...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103114/ https://www.ncbi.nlm.nih.gov/pubmed/25202628 http://dx.doi.org/10.3732/apps.1400026 |
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author | Yamauchi, Satoka Ohsako, Takanori |
author_facet | Yamauchi, Satoka Ohsako, Takanori |
author_sort | Yamauchi, Satoka |
collection | PubMed |
description | • Premise of the study: We developed microsatellite markers for Fimbristylis sericea, a coastal herb found in sand dunes in Asia and Australia. • Methods and Results: Twelve microsatellite loci were isolated, and the genetic variability within and among local populations was characterized. The number of alleles per locus was two to five with a mean of 3.5, total expected heterozygosity per locus was 0.069–0.645 with a mean of 0.336, and average expected heterozygosity within a population per locus was 0.051–0.230. Most of the loci deviated significantly from Hardy–Weinberg equilibrium. • Conclusions: All 12 microsatellite loci were polymorphic within and among populations. These loci could be useful genetic markers for population genetic studies of F. sericea populations. |
format | Online Article Text |
id | pubmed-4103114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41031142014-09-08 Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1) Yamauchi, Satoka Ohsako, Takanori Appl Plant Sci Primer Note • Premise of the study: We developed microsatellite markers for Fimbristylis sericea, a coastal herb found in sand dunes in Asia and Australia. • Methods and Results: Twelve microsatellite loci were isolated, and the genetic variability within and among local populations was characterized. The number of alleles per locus was two to five with a mean of 3.5, total expected heterozygosity per locus was 0.069–0.645 with a mean of 0.336, and average expected heterozygosity within a population per locus was 0.051–0.230. Most of the loci deviated significantly from Hardy–Weinberg equilibrium. • Conclusions: All 12 microsatellite loci were polymorphic within and among populations. These loci could be useful genetic markers for population genetic studies of F. sericea populations. Botanical Society of America 2014-05-07 /pmc/articles/PMC4103114/ /pubmed/25202628 http://dx.doi.org/10.3732/apps.1400026 Text en © 2014 Yamauchi and Ohsako. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Yamauchi, Satoka Ohsako, Takanori Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1) |
title | Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1) |
title_full | Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1) |
title_fullStr | Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1) |
title_full_unstemmed | Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1) |
title_short | Isolation and characterization of microsatellite loci in Fimbristylis sericea (Cyperaceae)(1) |
title_sort | isolation and characterization of microsatellite loci in fimbristylis sericea (cyperaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103114/ https://www.ncbi.nlm.nih.gov/pubmed/25202628 http://dx.doi.org/10.3732/apps.1400026 |
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