Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)(1)

• Premise of the study: Fifty microsatellite loci were developed for the endangered species Juglans mandshurica to investigate its genetic diversity and population structure. • Methods and Results: In all, 50 microsatellite markers were isolated from J. mandshurica, using the Fast Isolation by AFLP...

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Autores principales: Chen, Chang-Mei, Han, Shi-Jie, Yuan, Shu-Sheng, Wang, Chun-Jing, Yu, Jing-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103126/
https://www.ncbi.nlm.nih.gov/pubmed/25202563
http://dx.doi.org/10.3732/apps.1200009
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author Chen, Chang-Mei
Han, Shi-Jie
Yuan, Shu-Sheng
Wang, Chun-Jing
Yu, Jing-Hua
author_facet Chen, Chang-Mei
Han, Shi-Jie
Yuan, Shu-Sheng
Wang, Chun-Jing
Yu, Jing-Hua
author_sort Chen, Chang-Mei
collection PubMed
description • Premise of the study: Fifty microsatellite loci were developed for the endangered species Juglans mandshurica to investigate its genetic diversity and population structure. • Methods and Results: In all, 50 microsatellite markers were isolated from J. mandshurica, using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Twenty of these polymorphic markers were assessed in samples collected from 98 individuals among five populations in northeastern China. Across all of the J. mandshurica samples, the number of alleles per locus ranged from one to 17. • Conclusions: These new microsatellite loci will be useful for conservation genetics studies of J. mandshurica.
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spelling pubmed-41031262014-09-08 Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)(1) Chen, Chang-Mei Han, Shi-Jie Yuan, Shu-Sheng Wang, Chun-Jing Yu, Jing-Hua Appl Plant Sci Primer Note • Premise of the study: Fifty microsatellite loci were developed for the endangered species Juglans mandshurica to investigate its genetic diversity and population structure. • Methods and Results: In all, 50 microsatellite markers were isolated from J. mandshurica, using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Twenty of these polymorphic markers were assessed in samples collected from 98 individuals among five populations in northeastern China. Across all of the J. mandshurica samples, the number of alleles per locus ranged from one to 17. • Conclusions: These new microsatellite loci will be useful for conservation genetics studies of J. mandshurica. Botanical Society of America 2013-06-28 /pmc/articles/PMC4103126/ /pubmed/25202563 http://dx.doi.org/10.3732/apps.1200009 Text en © 2013 Chen et al. Published by the Botanical Society of America, Inc. http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Chen, Chang-Mei
Han, Shi-Jie
Yuan, Shu-Sheng
Wang, Chun-Jing
Yu, Jing-Hua
Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)(1)
title Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)(1)
title_full Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)(1)
title_fullStr Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)(1)
title_full_unstemmed Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)(1)
title_short Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)(1)
title_sort isolation and characterization of 20 polymorphic microsatellite markers for juglans mandshurica (juglandaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103126/
https://www.ncbi.nlm.nih.gov/pubmed/25202563
http://dx.doi.org/10.3732/apps.1200009
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