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Genus-wide microsatellite primers for the goldenrods (Solidago; Asteraceae)(1)

• Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus Solidago. • Methods and Results: Illumina sequencing of a shotgun library from S. gigantea identified ca. 1900 putative...

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Detalles Bibliográficos
Autores principales: Beck, James B., Semple, John C., Brull, Justin M., Lance, Stacey L., Phillips, Mai M., Hoot, Sara B., Meyer, Gretchen A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103136/
https://www.ncbi.nlm.nih.gov/pubmed/25202617
http://dx.doi.org/10.3732/apps.1300093
Descripción
Sumario:• Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus Solidago. • Methods and Results: Illumina sequencing of a shotgun library from S. gigantea identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of Solidago species. • Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecific studies in the ca. 120 Solidago species.