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Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)(1)
• Premise of the study: To facilitate genetic and conservation research of Cornus sanguinea, microsatellite loci were isolated and 29 individuals from 11 German populations were genotyped. • Methods and Results: Sixteen microsatellite loci were characterized from an enriched small insert genomic lib...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103144/ https://www.ncbi.nlm.nih.gov/pubmed/25202583 http://dx.doi.org/10.3732/apps.1300012 |
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author | Wadl, Phillip A. Hatmaker, E. Anne Fussi, Barbara Scheffler, Brian E. Trigiano, Robert N. |
author_facet | Wadl, Phillip A. Hatmaker, E. Anne Fussi, Barbara Scheffler, Brian E. Trigiano, Robert N. |
author_sort | Wadl, Phillip A. |
collection | PubMed |
description | • Premise of the study: To facilitate genetic and conservation research of Cornus sanguinea, microsatellite loci were isolated and 29 individuals from 11 German populations were genotyped. • Methods and Results: Sixteen microsatellite loci were characterized from an enriched small insert genomic library. The number of alleles detected ranged from five to 11 per locus, observed heterozygosity ranged from 0.00 to 1.00, expected heterozygosity ranged from 0.65 to 0.90, and polymorphic information content ranged from 0.59 to 0.88. • Conclusions: The markers described in the study will allow further investigation of population dynamics and the degree of clonal reproduction within populations of C. sanguinea. |
format | Online Article Text |
id | pubmed-4103144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41031442014-09-08 Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)(1) Wadl, Phillip A. Hatmaker, E. Anne Fussi, Barbara Scheffler, Brian E. Trigiano, Robert N. Appl Plant Sci Primer Note • Premise of the study: To facilitate genetic and conservation research of Cornus sanguinea, microsatellite loci were isolated and 29 individuals from 11 German populations were genotyped. • Methods and Results: Sixteen microsatellite loci were characterized from an enriched small insert genomic library. The number of alleles detected ranged from five to 11 per locus, observed heterozygosity ranged from 0.00 to 1.00, expected heterozygosity ranged from 0.65 to 0.90, and polymorphic information content ranged from 0.59 to 0.88. • Conclusions: The markers described in the study will allow further investigation of population dynamics and the degree of clonal reproduction within populations of C. sanguinea. Botanical Society of America 2013-09-02 /pmc/articles/PMC4103144/ /pubmed/25202583 http://dx.doi.org/10.3732/apps.1300012 Text en © 2013 Wadl et al. Published by the Botanical Society of America This article is a U.S. Government work and is in the public domain in the USA. |
spellingShingle | Primer Note Wadl, Phillip A. Hatmaker, E. Anne Fussi, Barbara Scheffler, Brian E. Trigiano, Robert N. Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)(1) |
title | Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)(1) |
title_full | Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)(1) |
title_fullStr | Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)(1) |
title_full_unstemmed | Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)(1) |
title_short | Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)(1) |
title_sort | isolation and characterization of microsatellite loci for cornus sanguinea (cornaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103144/ https://www.ncbi.nlm.nih.gov/pubmed/25202583 http://dx.doi.org/10.3732/apps.1300012 |
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