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Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)(1)
• Premise of the study: Microsatellite primers were identified and characterized in Acca sellowiana in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication. • Methods and Results: A...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103439/ https://www.ncbi.nlm.nih.gov/pubmed/25202632 http://dx.doi.org/10.3732/apps.1400020 |
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author | Klabunde, Gustavo H. F. Olkoski, Denise Vilperte, Vinicius Zucchi, Maria I. Nodari, Rubens O. |
author_facet | Klabunde, Gustavo H. F. Olkoski, Denise Vilperte, Vinicius Zucchi, Maria I. Nodari, Rubens O. |
author_sort | Klabunde, Gustavo H. F. |
collection | PubMed |
description | • Premise of the study: Microsatellite primers were identified and characterized in Acca sellowiana in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication. • Methods and Results: A total of 10 polymorphic microsatellite primers were designed from clones obtained from a simple sequence repeat (SSR)–enriched genomic library. The primers amplified di- and trinucleotide repeats with four to 27 alleles per locus. In all tested populations, the observed heterozygosity ranged from 0.269 to 1.0. • Conclusions: These new polymorphic SSR markers will allow future genetic studies to be denser, either for genetic structure characterization of natural populations or for studies involving genetic breeding and domestication process in A. sellowiana. |
format | Online Article Text |
id | pubmed-4103439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41034392014-09-08 Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)(1) Klabunde, Gustavo H. F. Olkoski, Denise Vilperte, Vinicius Zucchi, Maria I. Nodari, Rubens O. Appl Plant Sci Primer Note • Premise of the study: Microsatellite primers were identified and characterized in Acca sellowiana in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication. • Methods and Results: A total of 10 polymorphic microsatellite primers were designed from clones obtained from a simple sequence repeat (SSR)–enriched genomic library. The primers amplified di- and trinucleotide repeats with four to 27 alleles per locus. In all tested populations, the observed heterozygosity ranged from 0.269 to 1.0. • Conclusions: These new polymorphic SSR markers will allow future genetic studies to be denser, either for genetic structure characterization of natural populations or for studies involving genetic breeding and domestication process in A. sellowiana. Botanical Society of America 2014-06-04 /pmc/articles/PMC4103439/ /pubmed/25202632 http://dx.doi.org/10.3732/apps.1400020 Text en © 2014 Klabunde et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Klabunde, Gustavo H. F. Olkoski, Denise Vilperte, Vinicius Zucchi, Maria I. Nodari, Rubens O. Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)(1) |
title | Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)(1) |
title_full | Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)(1) |
title_fullStr | Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)(1) |
title_full_unstemmed | Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)(1) |
title_short | Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)(1) |
title_sort | characterization of 10 new nuclear microsatellite markers in acca sellowiana (myrtaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103439/ https://www.ncbi.nlm.nih.gov/pubmed/25202632 http://dx.doi.org/10.3732/apps.1400020 |
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