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Novel microsatellite markers for the high-alpine Geum reptans (Rosaceae)(1)
• Premise of the study: Geum reptans reproduces by outcrossing or by the formation of stolons. Sexual and clonal reproduction are not exclusive and occur mostly simultaneously. We developed novel microsatellite primers for this species, which will be used in a study about local adaptation, phenotypi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103440/ https://www.ncbi.nlm.nih.gov/pubmed/25202633 http://dx.doi.org/10.3732/apps.1400021 |
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author | Hamann, Elena Kesselring, Halil Stöcklin, Jürg Armbruster, Georg F. J. |
author_facet | Hamann, Elena Kesselring, Halil Stöcklin, Jürg Armbruster, Georg F. J. |
author_sort | Hamann, Elena |
collection | PubMed |
description | • Premise of the study: Geum reptans reproduces by outcrossing or by the formation of stolons. Sexual and clonal reproduction are not exclusive and occur mostly simultaneously. We developed novel microsatellite primers for this species, which will be used in a study about local adaptation, phenotypic plasticity, and random molecular divergence of alpine plants. • Methods and Results: Twelve microsatellite primer sets were developed for G. reptans, of which nine were polymorphic. Initially, the forward primers had an M13 tail, and the allelic signals of each locus were amplified using a single fluorescent-labeled M13 forward sequence. In the running phase, a multiplex PCR assay was developed using different fluorophore-labeled forward primers. Two to 11 alleles were found per locus, depending on the studied population. • Conclusions: Identical multilocus genotypes (i.e., clonal offspring) were not found because individuals in our sampling were at least 4 m distant from each other. F(ST)–Q(ST) analysis will be applied to detect selection processes in populations of G. reptans across the Alps. |
format | Online Article Text |
id | pubmed-4103440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41034402014-09-08 Novel microsatellite markers for the high-alpine Geum reptans (Rosaceae)(1) Hamann, Elena Kesselring, Halil Stöcklin, Jürg Armbruster, Georg F. J. Appl Plant Sci Primer Note • Premise of the study: Geum reptans reproduces by outcrossing or by the formation of stolons. Sexual and clonal reproduction are not exclusive and occur mostly simultaneously. We developed novel microsatellite primers for this species, which will be used in a study about local adaptation, phenotypic plasticity, and random molecular divergence of alpine plants. • Methods and Results: Twelve microsatellite primer sets were developed for G. reptans, of which nine were polymorphic. Initially, the forward primers had an M13 tail, and the allelic signals of each locus were amplified using a single fluorescent-labeled M13 forward sequence. In the running phase, a multiplex PCR assay was developed using different fluorophore-labeled forward primers. Two to 11 alleles were found per locus, depending on the studied population. • Conclusions: Identical multilocus genotypes (i.e., clonal offspring) were not found because individuals in our sampling were at least 4 m distant from each other. F(ST)–Q(ST) analysis will be applied to detect selection processes in populations of G. reptans across the Alps. Botanical Society of America 2014-06-04 /pmc/articles/PMC4103440/ /pubmed/25202633 http://dx.doi.org/10.3732/apps.1400021 Text en © 2014 Hamann et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Hamann, Elena Kesselring, Halil Stöcklin, Jürg Armbruster, Georg F. J. Novel microsatellite markers for the high-alpine Geum reptans (Rosaceae)(1) |
title | Novel microsatellite markers for the high-alpine Geum reptans (Rosaceae)(1) |
title_full | Novel microsatellite markers for the high-alpine Geum reptans (Rosaceae)(1) |
title_fullStr | Novel microsatellite markers for the high-alpine Geum reptans (Rosaceae)(1) |
title_full_unstemmed | Novel microsatellite markers for the high-alpine Geum reptans (Rosaceae)(1) |
title_short | Novel microsatellite markers for the high-alpine Geum reptans (Rosaceae)(1) |
title_sort | novel microsatellite markers for the high-alpine geum reptans (rosaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103440/ https://www.ncbi.nlm.nih.gov/pubmed/25202633 http://dx.doi.org/10.3732/apps.1400021 |
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