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Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa(1)

• Premise of the study: The North American genus Camassia is an ecologically important group whose variability and evolution are little understood, being influenced by hybridization and geographic isolation. We developed microsatellite markers to investigate patterns of gene flow, population structu...

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Autores principales: Culley, Theresa M., Leng, Ju-Fang, Kephart, Susan R., Cartieri, Francis J., Theiss, Kathryn E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103445/
https://www.ncbi.nlm.nih.gov/pubmed/25202572
http://dx.doi.org/10.3732/apps.1300001
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author Culley, Theresa M.
Leng, Ju-Fang
Kephart, Susan R.
Cartieri, Francis J.
Theiss, Kathryn E.
author_facet Culley, Theresa M.
Leng, Ju-Fang
Kephart, Susan R.
Cartieri, Francis J.
Theiss, Kathryn E.
author_sort Culley, Theresa M.
collection PubMed
description • Premise of the study: The North American genus Camassia is an ecologically important group whose variability and evolution are little understood, being influenced by hybridization and geographic isolation. We developed microsatellite markers to investigate patterns of gene flow, population structure, and taxonomic relationships within this group. • Methods and Results: Using a traditional approach with biotin-labeled probes, we developed 16 microsatellite primers in three species of Camassia: C. howellii, C. leichtlinii, and C. quamash. The number of alleles per locus averaged 3.94 per species, and levels of heterozygosity ranged from 0.000 to 1.00 and 0.033 to 0.917 for observed and expected heterozygosities, respectively. All primers amplified to varying extents in additional species (C. angusta, C. cusickii, C. scilloides) and in putative species in a related genus (Hastingsia alba, H. atropurpurea, H. bracteosa, H. serpentinicola). • Conclusions: These microsatellite markers exhibit variation and are useful for ongoing studies of integrative taxonomy and population differentiation within this species complex.
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spelling pubmed-41034452014-09-08 Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa(1) Culley, Theresa M. Leng, Ju-Fang Kephart, Susan R. Cartieri, Francis J. Theiss, Kathryn E. Appl Plant Sci Primer Note • Premise of the study: The North American genus Camassia is an ecologically important group whose variability and evolution are little understood, being influenced by hybridization and geographic isolation. We developed microsatellite markers to investigate patterns of gene flow, population structure, and taxonomic relationships within this group. • Methods and Results: Using a traditional approach with biotin-labeled probes, we developed 16 microsatellite primers in three species of Camassia: C. howellii, C. leichtlinii, and C. quamash. The number of alleles per locus averaged 3.94 per species, and levels of heterozygosity ranged from 0.000 to 1.00 and 0.033 to 0.917 for observed and expected heterozygosities, respectively. All primers amplified to varying extents in additional species (C. angusta, C. cusickii, C. scilloides) and in putative species in a related genus (Hastingsia alba, H. atropurpurea, H. bracteosa, H. serpentinicola). • Conclusions: These microsatellite markers exhibit variation and are useful for ongoing studies of integrative taxonomy and population differentiation within this species complex. Botanical Society of America 2013-07-22 /pmc/articles/PMC4103445/ /pubmed/25202572 http://dx.doi.org/10.3732/apps.1300001 Text en © 2013 Culley et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Culley, Theresa M.
Leng, Ju-Fang
Kephart, Susan R.
Cartieri, Francis J.
Theiss, Kathryn E.
Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa(1)
title Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa(1)
title_full Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa(1)
title_fullStr Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa(1)
title_full_unstemmed Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa(1)
title_short Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa(1)
title_sort development of 16 microsatellite markers within the camassia (agavaceae) species complex and amplification in related taxa(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103445/
https://www.ncbi.nlm.nih.gov/pubmed/25202572
http://dx.doi.org/10.3732/apps.1300001
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