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Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1)
• Premise of the study: To investigate population genetics, phylogeography, and cultivar origin of Ginkgo biloba, chloroplast microsatellite primers were developed. • Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chloroplast genomes...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103450/ https://www.ncbi.nlm.nih.gov/pubmed/25202577 http://dx.doi.org/10.3732/apps.1300019 |
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author | Xie, Chun-Xiang Zhao, Ming-Shui Fu, Cheng-Xin Zhao, Yun-Peng |
author_facet | Xie, Chun-Xiang Zhao, Ming-Shui Fu, Cheng-Xin Zhao, Yun-Peng |
author_sort | Xie, Chun-Xiang |
collection | PubMed |
description | • Premise of the study: To investigate population genetics, phylogeography, and cultivar origin of Ginkgo biloba, chloroplast microsatellite primers were developed. • Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chloroplast genomes of G. biloba. Polymorphisms were assessed on four natural populations from the two refugia in China. Eight loci were detected to be polymorphic in these populations. The number of alleles per locus ranged from three to seven, and the unbiased haploid diversity per locus varied from 0.441 to 0.807. • Conclusions: For the first time, we developed 21 chloroplast microsatellite markers for G. biloba, including 13 monomorphic and eight polymorphic ones within the assessed natural populations. These markers should provide a powerful tool for the study of genetic variation of both natural and cultivated populations of G. biloba, as well as cultivars. |
format | Online Article Text |
id | pubmed-4103450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41034502014-09-08 Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1) Xie, Chun-Xiang Zhao, Ming-Shui Fu, Cheng-Xin Zhao, Yun-Peng Appl Plant Sci Primer Note • Premise of the study: To investigate population genetics, phylogeography, and cultivar origin of Ginkgo biloba, chloroplast microsatellite primers were developed. • Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chloroplast genomes of G. biloba. Polymorphisms were assessed on four natural populations from the two refugia in China. Eight loci were detected to be polymorphic in these populations. The number of alleles per locus ranged from three to seven, and the unbiased haploid diversity per locus varied from 0.441 to 0.807. • Conclusions: For the first time, we developed 21 chloroplast microsatellite markers for G. biloba, including 13 monomorphic and eight polymorphic ones within the assessed natural populations. These markers should provide a powerful tool for the study of genetic variation of both natural and cultivated populations of G. biloba, as well as cultivars. Botanical Society of America 2013-07-29 /pmc/articles/PMC4103450/ /pubmed/25202577 http://dx.doi.org/10.3732/apps.1300019 Text en © 2013 Xie et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Xie, Chun-Xiang Zhao, Ming-Shui Fu, Cheng-Xin Zhao, Yun-Peng Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1) |
title | Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1) |
title_full | Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1) |
title_fullStr | Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1) |
title_full_unstemmed | Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1) |
title_short | Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1) |
title_sort | development of the first chloroplast microsatellite loci in ginkgo biloba (ginkgoaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103450/ https://www.ncbi.nlm.nih.gov/pubmed/25202577 http://dx.doi.org/10.3732/apps.1300019 |
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