Cargando…

Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1)

• Premise of the study: To investigate population genetics, phylogeography, and cultivar origin of Ginkgo biloba, chloroplast microsatellite primers were developed. • Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chloroplast genomes...

Descripción completa

Detalles Bibliográficos
Autores principales: Xie, Chun-Xiang, Zhao, Ming-Shui, Fu, Cheng-Xin, Zhao, Yun-Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103450/
https://www.ncbi.nlm.nih.gov/pubmed/25202577
http://dx.doi.org/10.3732/apps.1300019
_version_ 1782327145358426112
author Xie, Chun-Xiang
Zhao, Ming-Shui
Fu, Cheng-Xin
Zhao, Yun-Peng
author_facet Xie, Chun-Xiang
Zhao, Ming-Shui
Fu, Cheng-Xin
Zhao, Yun-Peng
author_sort Xie, Chun-Xiang
collection PubMed
description • Premise of the study: To investigate population genetics, phylogeography, and cultivar origin of Ginkgo biloba, chloroplast microsatellite primers were developed. • Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chloroplast genomes of G. biloba. Polymorphisms were assessed on four natural populations from the two refugia in China. Eight loci were detected to be polymorphic in these populations. The number of alleles per locus ranged from three to seven, and the unbiased haploid diversity per locus varied from 0.441 to 0.807. • Conclusions: For the first time, we developed 21 chloroplast microsatellite markers for G. biloba, including 13 monomorphic and eight polymorphic ones within the assessed natural populations. These markers should provide a powerful tool for the study of genetic variation of both natural and cultivated populations of G. biloba, as well as cultivars.
format Online
Article
Text
id pubmed-4103450
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Botanical Society of America
record_format MEDLINE/PubMed
spelling pubmed-41034502014-09-08 Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1) Xie, Chun-Xiang Zhao, Ming-Shui Fu, Cheng-Xin Zhao, Yun-Peng Appl Plant Sci Primer Note • Premise of the study: To investigate population genetics, phylogeography, and cultivar origin of Ginkgo biloba, chloroplast microsatellite primers were developed. • Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chloroplast genomes of G. biloba. Polymorphisms were assessed on four natural populations from the two refugia in China. Eight loci were detected to be polymorphic in these populations. The number of alleles per locus ranged from three to seven, and the unbiased haploid diversity per locus varied from 0.441 to 0.807. • Conclusions: For the first time, we developed 21 chloroplast microsatellite markers for G. biloba, including 13 monomorphic and eight polymorphic ones within the assessed natural populations. These markers should provide a powerful tool for the study of genetic variation of both natural and cultivated populations of G. biloba, as well as cultivars. Botanical Society of America 2013-07-29 /pmc/articles/PMC4103450/ /pubmed/25202577 http://dx.doi.org/10.3732/apps.1300019 Text en © 2013 Xie et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Xie, Chun-Xiang
Zhao, Ming-Shui
Fu, Cheng-Xin
Zhao, Yun-Peng
Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1)
title Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1)
title_full Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1)
title_fullStr Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1)
title_full_unstemmed Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1)
title_short Development of the first chloroplast microsatellite loci in Ginkgo biloba (Ginkgoaceae)(1)
title_sort development of the first chloroplast microsatellite loci in ginkgo biloba (ginkgoaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103450/
https://www.ncbi.nlm.nih.gov/pubmed/25202577
http://dx.doi.org/10.3732/apps.1300019
work_keys_str_mv AT xiechunxiang developmentofthefirstchloroplastmicrosatellitelociinginkgobilobaginkgoaceae1
AT zhaomingshui developmentofthefirstchloroplastmicrosatellitelociinginkgobilobaginkgoaceae1
AT fuchengxin developmentofthefirstchloroplastmicrosatellitelociinginkgobilobaginkgoaceae1
AT zhaoyunpeng developmentofthefirstchloroplastmicrosatellitelociinginkgobilobaginkgoaceae1