Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)(1)

• Premise of the study: Novel markers were developed for pea (Pisum sativum) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. • Methods and Results: Seventy-...

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Autores principales: Jain, Shalu, McPhee, Kevin E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103456/
https://www.ncbi.nlm.nih.gov/pubmed/25202494
http://dx.doi.org/10.3732/apps.1300026
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author Jain, Shalu
McPhee, Kevin E.
author_facet Jain, Shalu
McPhee, Kevin E.
author_sort Jain, Shalu
collection PubMed
description • Premise of the study: Novel markers were developed for pea (Pisum sativum) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. • Methods and Results: Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. • Conclusions: The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris) demonstrates their potential for use in closely related species.
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spelling pubmed-41034562014-09-08 Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)(1) Jain, Shalu McPhee, Kevin E. Appl Plant Sci Primer Note • Premise of the study: Novel markers were developed for pea (Pisum sativum) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. • Methods and Results: Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. • Conclusions: The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris) demonstrates their potential for use in closely related species. Botanical Society of America 2013-10-28 /pmc/articles/PMC4103456/ /pubmed/25202494 http://dx.doi.org/10.3732/apps.1300026 Text en © 2013 Jain and McPhee. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Jain, Shalu
McPhee, Kevin E.
Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)(1)
title Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)(1)
title_full Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)(1)
title_fullStr Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)(1)
title_full_unstemmed Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)(1)
title_short Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)(1)
title_sort isolation and characterization of novel est-derived genic markers in pisum sativum (fabaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103456/
https://www.ncbi.nlm.nih.gov/pubmed/25202494
http://dx.doi.org/10.3732/apps.1300026
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