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Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)(1)
• Premise of the study: This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. • Methods and Results: Microsatellite markers were developed using a microsatel...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103464/ https://www.ncbi.nlm.nih.gov/pubmed/25202484 http://dx.doi.org/10.3732/apps.1300015 |
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author | López-Roberts, M. Cristina de Queiroz, Luciano Paganucci van den Berg, Cássio |
author_facet | López-Roberts, M. Cristina de Queiroz, Luciano Paganucci van den Berg, Cássio |
author_sort | López-Roberts, M. Cristina |
collection | PubMed |
description | • Premise of the study: This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. • Methods and Results: Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 markers, 12 microsatellite loci were polymorphic and presented considerable variation in allele number (2–11), expected heterozygosity (0.226–0.883), and polymorphism information content per locus (0.212–0.870). Cross-amplification in C. argentea was successful in 16 loci, 12 of which were polymorphic (2–10 alleles). • Conclusions: The polymorphism of this set of microsatellite markers for C. mollis, as well as their successful cross-amplification in C. intermedia and C. bahiensis and their transferability to C. argentea, supports their use in future comparative studies to understand the mechanism involved in population divergence and speciation in the genus. |
format | Online Article Text |
id | pubmed-4103464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41034642014-09-08 Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)(1) López-Roberts, M. Cristina de Queiroz, Luciano Paganucci van den Berg, Cássio Appl Plant Sci Primer Note • Premise of the study: This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. • Methods and Results: Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 markers, 12 microsatellite loci were polymorphic and presented considerable variation in allele number (2–11), expected heterozygosity (0.226–0.883), and polymorphism information content per locus (0.212–0.870). Cross-amplification in C. argentea was successful in 16 loci, 12 of which were polymorphic (2–10 alleles). • Conclusions: The polymorphism of this set of microsatellite markers for C. mollis, as well as their successful cross-amplification in C. intermedia and C. bahiensis and their transferability to C. argentea, supports their use in future comparative studies to understand the mechanism involved in population divergence and speciation in the genus. Botanical Society of America 2013-10-04 /pmc/articles/PMC4103464/ /pubmed/25202484 http://dx.doi.org/10.3732/apps.1300015 Text en © 2013 López-Roberts et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note López-Roberts, M. Cristina de Queiroz, Luciano Paganucci van den Berg, Cássio Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)(1) |
title | Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)(1) |
title_full | Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)(1) |
title_fullStr | Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)(1) |
title_full_unstemmed | Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)(1) |
title_short | Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)(1) |
title_sort | development of microsatellite markers in cratylia mollis and their transferability to c. argentea (fabaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103464/ https://www.ncbi.nlm.nih.gov/pubmed/25202484 http://dx.doi.org/10.3732/apps.1300015 |
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