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Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1)

• Premise of the study: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • Methods and Results: Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism wit...

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Autores principales: Van Etten, Megan L., Robertson, Alastair W., Tate, Jennifer A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103472/
https://www.ncbi.nlm.nih.gov/pubmed/25202492
http://dx.doi.org/10.3732/apps.1300045
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author Van Etten, Megan L.
Robertson, Alastair W.
Tate, Jennifer A.
author_facet Van Etten, Megan L.
Robertson, Alastair W.
Tate, Jennifer A.
author_sort Van Etten, Megan L.
collection PubMed
description • Premise of the study: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • Methods and Results: Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymorphic within at least three populations and consistently amplified in the four populations tested. These primers amplified di-, tri-, and tetranucleotide repeats with 1–10 alleles per population. • Conclusions: These results indicate the utility of microsatellite loci for future mating system and population genetic studies in F. excorticata.
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spelling pubmed-41034722014-09-08 Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1) Van Etten, Megan L. Robertson, Alastair W. Tate, Jennifer A. Appl Plant Sci Primer Note • Premise of the study: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • Methods and Results: Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymorphic within at least three populations and consistently amplified in the four populations tested. These primers amplified di-, tri-, and tetranucleotide repeats with 1–10 alleles per population. • Conclusions: These results indicate the utility of microsatellite loci for future mating system and population genetic studies in F. excorticata. Botanical Society of America 2013-10-01 /pmc/articles/PMC4103472/ /pubmed/25202492 http://dx.doi.org/10.3732/apps.1300045 Text en © 2013 Van Etten et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Van Etten, Megan L.
Robertson, Alastair W.
Tate, Jennifer A.
Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1)
title Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1)
title_full Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1)
title_fullStr Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1)
title_full_unstemmed Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1)
title_short Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1)
title_sort microsatellite markers for the new zealand endemic tree fuchsia excorticata (onagraceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103472/
https://www.ncbi.nlm.nih.gov/pubmed/25202492
http://dx.doi.org/10.3732/apps.1300045
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AT tatejennifera microsatellitemarkersforthenewzealandendemictreefuchsiaexcorticataonagraceae1