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Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1)
• Premise of the study: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • Methods and Results: Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism wit...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103472/ https://www.ncbi.nlm.nih.gov/pubmed/25202492 http://dx.doi.org/10.3732/apps.1300045 |
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author | Van Etten, Megan L. Robertson, Alastair W. Tate, Jennifer A. |
author_facet | Van Etten, Megan L. Robertson, Alastair W. Tate, Jennifer A. |
author_sort | Van Etten, Megan L. |
collection | PubMed |
description | • Premise of the study: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • Methods and Results: Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymorphic within at least three populations and consistently amplified in the four populations tested. These primers amplified di-, tri-, and tetranucleotide repeats with 1–10 alleles per population. • Conclusions: These results indicate the utility of microsatellite loci for future mating system and population genetic studies in F. excorticata. |
format | Online Article Text |
id | pubmed-4103472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41034722014-09-08 Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1) Van Etten, Megan L. Robertson, Alastair W. Tate, Jennifer A. Appl Plant Sci Primer Note • Premise of the study: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • Methods and Results: Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymorphic within at least three populations and consistently amplified in the four populations tested. These primers amplified di-, tri-, and tetranucleotide repeats with 1–10 alleles per population. • Conclusions: These results indicate the utility of microsatellite loci for future mating system and population genetic studies in F. excorticata. Botanical Society of America 2013-10-01 /pmc/articles/PMC4103472/ /pubmed/25202492 http://dx.doi.org/10.3732/apps.1300045 Text en © 2013 Van Etten et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Van Etten, Megan L. Robertson, Alastair W. Tate, Jennifer A. Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1) |
title | Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1) |
title_full | Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1) |
title_fullStr | Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1) |
title_full_unstemmed | Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1) |
title_short | Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae)(1) |
title_sort | microsatellite markers for the new zealand endemic tree fuchsia excorticata (onagraceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103472/ https://www.ncbi.nlm.nih.gov/pubmed/25202492 http://dx.doi.org/10.3732/apps.1300045 |
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