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Microsatellite-Primed PCR for Intra-species Genetic Relatedness in Trichophyton ajelloi Strains Isolated in Poland from Various Soil Samples

Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains...

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Autores principales: Ciesielska, Anita, Bohacz, Justyna, Korniłłowicz-Kowalska, Teresa, Stączek, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103524/
https://www.ncbi.nlm.nih.gov/pubmed/24859370
http://dx.doi.org/10.1264/jsme2.ME13160
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author Ciesielska, Anita
Bohacz, Justyna
Korniłłowicz-Kowalska, Teresa
Stączek, Paweł
author_facet Ciesielska, Anita
Bohacz, Justyna
Korniłłowicz-Kowalska, Teresa
Stączek, Paweł
author_sort Ciesielska, Anita
collection PubMed
description Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains isolated from 3 types of soils with different physicochemical properties. The first method, employed for molecular identification, was PCR amplification of the 5.8S rRNA gene and its flanking regions encoding internal transcribed spacers (ITSs), followed by restriction enzyme digestion using endonuclease HinfI. The second method, employed for molecular differentiation, was microsatellite-primed PCR (MSP-PCR) using the repetitive oligonucleotide (GACA)(4). All the T. ajelloi strains were also identified using a traditional culture method. Our results showed that molecular identification using the PCR-restriction fragment length polymorphism (PCR-RFLP) method agreed with the identification made using the traditional approach. On the other hand, PCR-RFLP results showed no strain differentiation, while MSP-PCR using the (GACA)(4) primer identified different varieties among the T. ajelloi strains. The reasons for the intra-species differentiation of T. ajelloi have been discussed.
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spelling pubmed-41035242014-07-24 Microsatellite-Primed PCR for Intra-species Genetic Relatedness in Trichophyton ajelloi Strains Isolated in Poland from Various Soil Samples Ciesielska, Anita Bohacz, Justyna Korniłłowicz-Kowalska, Teresa Stączek, Paweł Microbes Environ Articles Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains isolated from 3 types of soils with different physicochemical properties. The first method, employed for molecular identification, was PCR amplification of the 5.8S rRNA gene and its flanking regions encoding internal transcribed spacers (ITSs), followed by restriction enzyme digestion using endonuclease HinfI. The second method, employed for molecular differentiation, was microsatellite-primed PCR (MSP-PCR) using the repetitive oligonucleotide (GACA)(4). All the T. ajelloi strains were also identified using a traditional culture method. Our results showed that molecular identification using the PCR-restriction fragment length polymorphism (PCR-RFLP) method agreed with the identification made using the traditional approach. On the other hand, PCR-RFLP results showed no strain differentiation, while MSP-PCR using the (GACA)(4) primer identified different varieties among the T. ajelloi strains. The reasons for the intra-species differentiation of T. ajelloi have been discussed. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2014-06 2014-05-24 /pmc/articles/PMC4103524/ /pubmed/24859370 http://dx.doi.org/10.1264/jsme2.ME13160 Text en Copyright 2014 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Ciesielska, Anita
Bohacz, Justyna
Korniłłowicz-Kowalska, Teresa
Stączek, Paweł
Microsatellite-Primed PCR for Intra-species Genetic Relatedness in Trichophyton ajelloi Strains Isolated in Poland from Various Soil Samples
title Microsatellite-Primed PCR for Intra-species Genetic Relatedness in Trichophyton ajelloi Strains Isolated in Poland from Various Soil Samples
title_full Microsatellite-Primed PCR for Intra-species Genetic Relatedness in Trichophyton ajelloi Strains Isolated in Poland from Various Soil Samples
title_fullStr Microsatellite-Primed PCR for Intra-species Genetic Relatedness in Trichophyton ajelloi Strains Isolated in Poland from Various Soil Samples
title_full_unstemmed Microsatellite-Primed PCR for Intra-species Genetic Relatedness in Trichophyton ajelloi Strains Isolated in Poland from Various Soil Samples
title_short Microsatellite-Primed PCR for Intra-species Genetic Relatedness in Trichophyton ajelloi Strains Isolated in Poland from Various Soil Samples
title_sort microsatellite-primed pcr for intra-species genetic relatedness in trichophyton ajelloi strains isolated in poland from various soil samples
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103524/
https://www.ncbi.nlm.nih.gov/pubmed/24859370
http://dx.doi.org/10.1264/jsme2.ME13160
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