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Diversification of Bacterial Community Composition along a Temperature Gradient at a Thermal Spring
To better understand the biogeography and relationship between temperature and community structure within microbial mats, the bacterial diversity of mats at a slightly alkaline, sulfide-containing hot spring was explored. Microbial mats that developed at temperatures between 75–52°C were collected f...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103544/ https://www.ncbi.nlm.nih.gov/pubmed/22673306 http://dx.doi.org/10.1264/jsme2.ME11350 |
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author | Everroad, R. Craig Otaki, Hiroyo Matsuura, Katsumi Haruta, Shin |
author_facet | Everroad, R. Craig Otaki, Hiroyo Matsuura, Katsumi Haruta, Shin |
author_sort | Everroad, R. Craig |
collection | PubMed |
description | To better understand the biogeography and relationship between temperature and community structure within microbial mats, the bacterial diversity of mats at a slightly alkaline, sulfide-containing hot spring was explored. Microbial mats that developed at temperatures between 75–52°C were collected from an area of approximately 1 m(2) in Nakabusa, Nagano, Japan. Bacterial 16S rRNA genes from these samples were examined by terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis. T-RFLP profiles revealed 66 unique fragments (T-RFs). Based on total T-RFs observed in environmental profiles and clone libraries, a temperature effect on diversity was determined, with complexity in the community increasing as temperature decreased. The T-RF pattern indicated four distinct community assemblages related to temperature. Members of the Aquificales and particularly the sulfuroxidizing bacterium Sulfurihydrogenibium were present at all temperatures and were the dominant component of mats taken at 75–67°C. Sulfide oxidation, which persisted throughout the temperature gradient, was the presumed dominant pathway of primary production above 67°C. As temperature decreased, successive additions of anoxygenic and oxygenic phototrophs increased primary productivity, allowing for diversification of the community. |
format | Online Article Text |
id | pubmed-4103544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41035442014-07-24 Diversification of Bacterial Community Composition along a Temperature Gradient at a Thermal Spring Everroad, R. Craig Otaki, Hiroyo Matsuura, Katsumi Haruta, Shin Microbes Environ Articles To better understand the biogeography and relationship between temperature and community structure within microbial mats, the bacterial diversity of mats at a slightly alkaline, sulfide-containing hot spring was explored. Microbial mats that developed at temperatures between 75–52°C were collected from an area of approximately 1 m(2) in Nakabusa, Nagano, Japan. Bacterial 16S rRNA genes from these samples were examined by terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis. T-RFLP profiles revealed 66 unique fragments (T-RFs). Based on total T-RFs observed in environmental profiles and clone libraries, a temperature effect on diversity was determined, with complexity in the community increasing as temperature decreased. The T-RF pattern indicated four distinct community assemblages related to temperature. Members of the Aquificales and particularly the sulfuroxidizing bacterium Sulfurihydrogenibium were present at all temperatures and were the dominant component of mats taken at 75–67°C. Sulfide oxidation, which persisted throughout the temperature gradient, was the presumed dominant pathway of primary production above 67°C. As temperature decreased, successive additions of anoxygenic and oxygenic phototrophs increased primary productivity, allowing for diversification of the community. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012-12 2012-05-17 /pmc/articles/PMC4103544/ /pubmed/22673306 http://dx.doi.org/10.1264/jsme2.ME11350 Text en Copyright © 2012 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Everroad, R. Craig Otaki, Hiroyo Matsuura, Katsumi Haruta, Shin Diversification of Bacterial Community Composition along a Temperature Gradient at a Thermal Spring |
title | Diversification of Bacterial Community Composition along a Temperature Gradient at a Thermal Spring |
title_full | Diversification of Bacterial Community Composition along a Temperature Gradient at a Thermal Spring |
title_fullStr | Diversification of Bacterial Community Composition along a Temperature Gradient at a Thermal Spring |
title_full_unstemmed | Diversification of Bacterial Community Composition along a Temperature Gradient at a Thermal Spring |
title_short | Diversification of Bacterial Community Composition along a Temperature Gradient at a Thermal Spring |
title_sort | diversification of bacterial community composition along a temperature gradient at a thermal spring |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103544/ https://www.ncbi.nlm.nih.gov/pubmed/22673306 http://dx.doi.org/10.1264/jsme2.ME11350 |
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