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Characterization of Nitric Oxide-Inducing Lipid A Derived from Mesorhizobium loti Lipopolysaccharide
Mesorhizobium loti is a member of the rhizobia and forms nitrogen-fixing symbioses with several Lotus species. Recently, it was reported that M. loti bacterial cells and their lipopolysaccharide (LPS) preparations transiently induced nitric oxide (NO) production in the roots of L. japonicus. We subs...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103559/ https://www.ncbi.nlm.nih.gov/pubmed/23059724 http://dx.doi.org/10.1264/jsme2.ME12103 |
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author | Hashimoto, Masahito Tanishita, Youhei Suda, Yasuo Murakami, Ei-ichi Nagata, Maki Kucho, Ken-ichi Abe, Mikiko Uchiumi, Toshiki |
author_facet | Hashimoto, Masahito Tanishita, Youhei Suda, Yasuo Murakami, Ei-ichi Nagata, Maki Kucho, Ken-ichi Abe, Mikiko Uchiumi, Toshiki |
author_sort | Hashimoto, Masahito |
collection | PubMed |
description | Mesorhizobium loti is a member of the rhizobia and forms nitrogen-fixing symbioses with several Lotus species. Recently, it was reported that M. loti bacterial cells and their lipopolysaccharide (LPS) preparations transiently induced nitric oxide (NO) production in the roots of L. japonicus. We subsequently found that polysaccharides and the lipid A moiety were responsible for this NO induction. In this study, we elucidated the chemical structure of M. loti lipid A and characterized its NO-inducing activity in response to structural modifications. M. loti LPS were partially hydrolyzed with hydrazine or aqueous hydrofluoric acid to obtain O-deacylated or dephosphorylated LPS, respectively. The untreated and treated LPS fractions were subjected to weak acid hydrolysis to obtain lipid A fractions. The chemical structure of M. loti lipid A was elucidated by chemical composition analysis, MALDI-TOF-MS, and NMR spectra to be P-4-β-GlcNN(1-6)α-GlcNN(1-1)α-GalA, in which positions 2 and 3 of β-GlcNN are substituted for 3-acyloxy-fatty amides, and positions 2 and 3 of α-GlcNN are substituted for 3OH-fatty amides. The partial hydrolysis of lipid A appeared to reduce its NO-inducing activity. These results suggest that L. japonicus root cells recognize the lipid A structure as a means of controlling NO production. |
format | Online Article Text |
id | pubmed-4103559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41035592014-07-24 Characterization of Nitric Oxide-Inducing Lipid A Derived from Mesorhizobium loti Lipopolysaccharide Hashimoto, Masahito Tanishita, Youhei Suda, Yasuo Murakami, Ei-ichi Nagata, Maki Kucho, Ken-ichi Abe, Mikiko Uchiumi, Toshiki Microbes Environ Articles Mesorhizobium loti is a member of the rhizobia and forms nitrogen-fixing symbioses with several Lotus species. Recently, it was reported that M. loti bacterial cells and their lipopolysaccharide (LPS) preparations transiently induced nitric oxide (NO) production in the roots of L. japonicus. We subsequently found that polysaccharides and the lipid A moiety were responsible for this NO induction. In this study, we elucidated the chemical structure of M. loti lipid A and characterized its NO-inducing activity in response to structural modifications. M. loti LPS were partially hydrolyzed with hydrazine or aqueous hydrofluoric acid to obtain O-deacylated or dephosphorylated LPS, respectively. The untreated and treated LPS fractions were subjected to weak acid hydrolysis to obtain lipid A fractions. The chemical structure of M. loti lipid A was elucidated by chemical composition analysis, MALDI-TOF-MS, and NMR spectra to be P-4-β-GlcNN(1-6)α-GlcNN(1-1)α-GalA, in which positions 2 and 3 of β-GlcNN are substituted for 3-acyloxy-fatty amides, and positions 2 and 3 of α-GlcNN are substituted for 3OH-fatty amides. The partial hydrolysis of lipid A appeared to reduce its NO-inducing activity. These results suggest that L. japonicus root cells recognize the lipid A structure as a means of controlling NO production. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012-12 2012-10-10 /pmc/articles/PMC4103559/ /pubmed/23059724 http://dx.doi.org/10.1264/jsme2.ME12103 Text en Copyright © 2012 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Hashimoto, Masahito Tanishita, Youhei Suda, Yasuo Murakami, Ei-ichi Nagata, Maki Kucho, Ken-ichi Abe, Mikiko Uchiumi, Toshiki Characterization of Nitric Oxide-Inducing Lipid A Derived from Mesorhizobium loti Lipopolysaccharide |
title | Characterization of Nitric Oxide-Inducing Lipid A Derived from Mesorhizobium loti Lipopolysaccharide |
title_full | Characterization of Nitric Oxide-Inducing Lipid A Derived from Mesorhizobium loti Lipopolysaccharide |
title_fullStr | Characterization of Nitric Oxide-Inducing Lipid A Derived from Mesorhizobium loti Lipopolysaccharide |
title_full_unstemmed | Characterization of Nitric Oxide-Inducing Lipid A Derived from Mesorhizobium loti Lipopolysaccharide |
title_short | Characterization of Nitric Oxide-Inducing Lipid A Derived from Mesorhizobium loti Lipopolysaccharide |
title_sort | characterization of nitric oxide-inducing lipid a derived from mesorhizobium loti lipopolysaccharide |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103559/ https://www.ncbi.nlm.nih.gov/pubmed/23059724 http://dx.doi.org/10.1264/jsme2.ME12103 |
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