Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres

This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracyclin...

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Autores principales: Klibi, Naouel, Ben Slimen, Naouel, Fhoula, Imen, López, Maria, Ben Slama, Karim, Daffonchio, Daniele, Boudabous, Abdellatif, Torres, Carmen, Ouzari, Hadda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103568/
https://www.ncbi.nlm.nih.gov/pubmed/23124764
http://dx.doi.org/10.1264/jsme2.ME12041
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author Klibi, Naouel
Ben Slimen, Naouel
Fhoula, Imen
López, Maria
Ben Slama, Karim
Daffonchio, Daniele
Boudabous, Abdellatif
Torres, Carmen
Ouzari, Hadda
author_facet Klibi, Naouel
Ben Slimen, Naouel
Fhoula, Imen
López, Maria
Ben Slama, Karim
Daffonchio, Daniele
Boudabous, Abdellatif
Torres, Carmen
Ouzari, Hadda
author_sort Klibi, Naouel
collection PubMed
description This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.
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spelling pubmed-41035682014-07-24 Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres Klibi, Naouel Ben Slimen, Naouel Fhoula, Imen López, Maria Ben Slama, Karim Daffonchio, Daniele Boudabous, Abdellatif Torres, Carmen Ouzari, Hadda Microbes Environ Short Communication This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012-12 2012-10-31 /pmc/articles/PMC4103568/ /pubmed/23124764 http://dx.doi.org/10.1264/jsme2.ME12041 Text en Copyright © 2012 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Klibi, Naouel
Ben Slimen, Naouel
Fhoula, Imen
López, Maria
Ben Slama, Karim
Daffonchio, Daniele
Boudabous, Abdellatif
Torres, Carmen
Ouzari, Hadda
Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres
title Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres
title_full Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres
title_fullStr Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres
title_full_unstemmed Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres
title_short Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres
title_sort genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103568/
https://www.ncbi.nlm.nih.gov/pubmed/23124764
http://dx.doi.org/10.1264/jsme2.ME12041
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