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Behavioral and Cardiorespiratory Responses to Bilateral Microinjections of Oxytocin into the Central Nucleus of Amygdala of Wistar Rats, an Experimental Model of Compulsion
INTRODUCTION: The central nucleus of amygdala plays an important role mediating fear and anxiety responses. It is known that oxytocin microinjections into the central nucleus of amygdala induce hypergrooming, an experimental model of compulsive behavior. We evaluated the behavioral and cardiorespira...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103777/ https://www.ncbi.nlm.nih.gov/pubmed/25036025 http://dx.doi.org/10.1371/journal.pone.0099284 |
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author | Granjeiro, Érica Maria Marroni, Simone Saldanha Martins Dias, Daniel Penteado Heck Bonagamba, Leni Gomes Costa, Kauê Machado dos Santos, Jéssica Cristina Oliveira, José Antônio Cortes Machado, Benedito H. Garcia-Cairasco, Norberto |
author_facet | Granjeiro, Érica Maria Marroni, Simone Saldanha Martins Dias, Daniel Penteado Heck Bonagamba, Leni Gomes Costa, Kauê Machado dos Santos, Jéssica Cristina Oliveira, José Antônio Cortes Machado, Benedito H. Garcia-Cairasco, Norberto |
author_sort | Granjeiro, Érica Maria |
collection | PubMed |
description | INTRODUCTION: The central nucleus of amygdala plays an important role mediating fear and anxiety responses. It is known that oxytocin microinjections into the central nucleus of amygdala induce hypergrooming, an experimental model of compulsive behavior. We evaluated the behavioral and cardiorespiratory responses of conscious rats microinjected with oxytocin into the central nucleus of amygdala. METHODS: Male Wistar rats were implanted with guide cannulae into the central nucleus of amygdala and microinjected with oxytocin (0.5 µg, 1 µg) or saline. After 24 h, rats had a catheter implanted into the femoral artery for pulsatile arterial pressure measurement. The pulsatile arterial pressure was recorded at baseline conditions and data used for cardiovascular variability and baroreflex sensitivity analysis. Respiratory and behavioral parameters were assessed during this data collection session. RESULTS: Microinjections of oxytocin (0.5 µg) into the central nucleus of amygdala produced hypergrooming behavior but did not change cardiorespiratory parameters. However, hypergrooming evoked by microinjections of oxytocin (1 µg) into the central nucleus of amygdala was accompanied by increase in arterial pressure, heart rate and ventilation and augmented the power of low and high (respiratory-related) frequency bands of the systolic arterial pressure spectrum. No changes were observed in power of the low and high frequency bands of the pulse interval spectrum. Baroreflex sensitivity was found lower after oxytocin microinjections, demonstrating that the oxytocin-induced pressor response may involve an inhibition of baroreflex pathways and a consequent facilitation of sympathetic outflow to the cardiovascular system. CONCLUSIONS: The microinjection of oxytocin (1 µg) into the central nucleus of amygdala not only induces hypergrooming but also changes cardiorespiratory parameters. Moreover, specific oxytocin receptor antagonism attenuated hypergrooming but did not affect pressor, tachycardic and ventilatory responses to oxytocin, suggesting the involvement of distinct neural pathways. |
format | Online Article Text |
id | pubmed-4103777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41037772014-07-21 Behavioral and Cardiorespiratory Responses to Bilateral Microinjections of Oxytocin into the Central Nucleus of Amygdala of Wistar Rats, an Experimental Model of Compulsion Granjeiro, Érica Maria Marroni, Simone Saldanha Martins Dias, Daniel Penteado Heck Bonagamba, Leni Gomes Costa, Kauê Machado dos Santos, Jéssica Cristina Oliveira, José Antônio Cortes Machado, Benedito H. Garcia-Cairasco, Norberto PLoS One Research Article INTRODUCTION: The central nucleus of amygdala plays an important role mediating fear and anxiety responses. It is known that oxytocin microinjections into the central nucleus of amygdala induce hypergrooming, an experimental model of compulsive behavior. We evaluated the behavioral and cardiorespiratory responses of conscious rats microinjected with oxytocin into the central nucleus of amygdala. METHODS: Male Wistar rats were implanted with guide cannulae into the central nucleus of amygdala and microinjected with oxytocin (0.5 µg, 1 µg) or saline. After 24 h, rats had a catheter implanted into the femoral artery for pulsatile arterial pressure measurement. The pulsatile arterial pressure was recorded at baseline conditions and data used for cardiovascular variability and baroreflex sensitivity analysis. Respiratory and behavioral parameters were assessed during this data collection session. RESULTS: Microinjections of oxytocin (0.5 µg) into the central nucleus of amygdala produced hypergrooming behavior but did not change cardiorespiratory parameters. However, hypergrooming evoked by microinjections of oxytocin (1 µg) into the central nucleus of amygdala was accompanied by increase in arterial pressure, heart rate and ventilation and augmented the power of low and high (respiratory-related) frequency bands of the systolic arterial pressure spectrum. No changes were observed in power of the low and high frequency bands of the pulse interval spectrum. Baroreflex sensitivity was found lower after oxytocin microinjections, demonstrating that the oxytocin-induced pressor response may involve an inhibition of baroreflex pathways and a consequent facilitation of sympathetic outflow to the cardiovascular system. CONCLUSIONS: The microinjection of oxytocin (1 µg) into the central nucleus of amygdala not only induces hypergrooming but also changes cardiorespiratory parameters. Moreover, specific oxytocin receptor antagonism attenuated hypergrooming but did not affect pressor, tachycardic and ventilatory responses to oxytocin, suggesting the involvement of distinct neural pathways. Public Library of Science 2014-07-18 /pmc/articles/PMC4103777/ /pubmed/25036025 http://dx.doi.org/10.1371/journal.pone.0099284 Text en © 2014 Granjeiro et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Granjeiro, Érica Maria Marroni, Simone Saldanha Martins Dias, Daniel Penteado Heck Bonagamba, Leni Gomes Costa, Kauê Machado dos Santos, Jéssica Cristina Oliveira, José Antônio Cortes Machado, Benedito H. Garcia-Cairasco, Norberto Behavioral and Cardiorespiratory Responses to Bilateral Microinjections of Oxytocin into the Central Nucleus of Amygdala of Wistar Rats, an Experimental Model of Compulsion |
title | Behavioral and Cardiorespiratory Responses to Bilateral Microinjections of Oxytocin into the Central Nucleus of Amygdala of Wistar Rats, an Experimental Model of Compulsion |
title_full | Behavioral and Cardiorespiratory Responses to Bilateral Microinjections of Oxytocin into the Central Nucleus of Amygdala of Wistar Rats, an Experimental Model of Compulsion |
title_fullStr | Behavioral and Cardiorespiratory Responses to Bilateral Microinjections of Oxytocin into the Central Nucleus of Amygdala of Wistar Rats, an Experimental Model of Compulsion |
title_full_unstemmed | Behavioral and Cardiorespiratory Responses to Bilateral Microinjections of Oxytocin into the Central Nucleus of Amygdala of Wistar Rats, an Experimental Model of Compulsion |
title_short | Behavioral and Cardiorespiratory Responses to Bilateral Microinjections of Oxytocin into the Central Nucleus of Amygdala of Wistar Rats, an Experimental Model of Compulsion |
title_sort | behavioral and cardiorespiratory responses to bilateral microinjections of oxytocin into the central nucleus of amygdala of wistar rats, an experimental model of compulsion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103777/ https://www.ncbi.nlm.nih.gov/pubmed/25036025 http://dx.doi.org/10.1371/journal.pone.0099284 |
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